摘要
目的建立高效液相色谱法测定罗布麻定时脉冲片中5种酚酸及黄酮类成分含量的方法。方法采用Hypersil C18(4.6 mm×200 mm)色谱柱,乙腈-10 mmol.L-1醋酸铵缓冲液(pH 4,醋酸调节)为流动相,洗脱程序:0 min,10%乙腈;8 min,15%乙腈;16 min,17%乙腈;40 min,38%乙腈。流速1.0 mL.min-1。检测波长为256 nm。结果 2种酚酸及3种黄酮成分的峰面积与浓度的线性关系良好(r>0.999 0);加样回收率为(94.3±1.80)%～(102.4±2.89)%。结论该方法简单、准确,重现性较好,可用于罗布麻定时脉冲片内在质量的控制和评价。
OBJECTIVE To establish a method for determination of 5 caffeoylquinic acids and avonoids in Luobuma time-controlled Pulse tablet by HPLC.METHODS The HPLC method was performed on a Hypersil C18 column（4.6 mm×200 mm,5 μm）.The mobile phase was composed of acetonitril-10 mmol·L-1 ammonium acetate aqeous solution（pH 4.0,adjusted with acetic acid solution） at a flow rate of 1 mL·min-1.The gradient elution procedure was as follows： 0 min,10% acetonitril,8 min,15% acetonitril,16 min,17% acetonitril,40 min,38% acetonitril.The UV detection wavelength was 254 nm.RESULTS The calibration curves for the 2 caffeoylquinic acids and 3 flavonoids showed good linearity（r0.999 0） in the range of the tested concentrations.The average recoveries of the 5 compounds were in the range of（94.3±1.80）%-（102.4±2.89）%.CONCLUSION The method is simple,accurate and reproducible,and can be used to control and evaluate the quality of Luobuma time-controlled pulse tablet.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第20期1605-1608,共4页
Chinese Pharmaceutical Journal
基金
"十一五"重大新药创制资助项目"中药新型给药系统技术平台"(2009ZX09310-005)
关键词
罗布麻叶
定时脉冲给药系统
酚酸成分
黄酮类成分
含量测定
Apocynum venetum L.
time-controlled pulsatile drug delivery system
phenolic acids
flavonoids
determination
