摘要
目的:研究海南特有药用植物青皮Vatica mangachapoi Blanco的抗氧化活性。方法:利用加速溶剂萃取技术(ASE)快速制备青皮石油醚(MSO)、乙酸乙酯(EtOAc)、乙醇(EtOH)、甲醇(MeOH)提取物;结合酶标仪,快速筛选具有清除二苯代苦味肼基自由基(DPPH.)能力的ASE提取物。结果:青皮不同溶剂ASE提取物清除DPPH.的能力大小为:乙酸乙酯ASE提取物>乙醇ASE提取物>石油醚ASE提取物>甲醇ASE提取物;其IC50值分别为0.143 4,0.501 8,0.567 7,0.693 7 g.L-1。乙酸乙酯ASE提取物的抗氧化活性强于抗坏血酸(IC50=0.203 4 g.L-1)。结论:乙酸乙酯ASE提取物具有较强清除DPPH.的能力,有望开发成天然的抗氧化剂。
Objective：To investigate the antioxidant effect of medicinal plants Vatica mangachapoi in Hainan province.Method： The sample extractions were prepared by accelerated solvent extraction（ASE） using solvent petroleum ether（MSO）,ethyl acetate（EtOAc）,ethanol（EtOH）,methanol（MeOH）;Combining with microplate assay model,the ASEs that had high antioxidant activity were screened quickly.Result： The antioxidant activity of different solvent ASEs from Vatica mangachapoi was evaluated by DPPH assay.The antioxidant activity was listed as follows： ethyl acetateethanolpetroleum ethermethanol;its IC50 values were 0.143 4,0.501 8,0.567 7,0.693 7 g·L-1 respectively.The antioxidant of ethyl acetate ASE was better than ascorbic acid（IC50=0.203 4 g·L-1）.Conclusion： The ethyl acetate ASE showed great DPPH scavenging activity,it＇s hopeful to develop a new natural antioxidant.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第21期69-73,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家高新技术研究发展计划(863)项目(2007AA021500)
关键词
青皮
加速溶剂萃取
DPPH.
抗氧化
Vatica mangachapoi
accelerated solvent extraction
DPPH
antioxidant
