摘要
[目的]探索赤霉素GA4+7在5000 L发酵罐中的中试发酵工艺条件。[方法]赤霉素GA4+7发酵培养基配方和发酵条件的优化筛选研究,分别在摇瓶条件下和在5000 L发酵罐中进行,采用多因素不同水平的正交试验设计。赤霉素GA4+7发酵单位的检测采用HPLC法。[结果]获得了赤霉素GA4+7发酵培养基优化配方:淀粉+葡萄糖为9.0%+1.0%、花生粉+黄豆粉为1.2%+0.6%、磷酸二氢钾和硫酸铵为0.2%和0.01%;获得了赤霉素GA4+7在5 000 L发酵罐中优化的发酵条件:温度32℃、发酵通气量V(空气)∶V(发酵液)为0.4∶1、种子罐接种摇瓶种液400 mL、种子罐培养液中硫酸铵含量为0.05%、发酵罐补糖、种子罐种龄和发酵移种量分别为38 h和12%、发酵罐中添加碳酸钙量为0.2%。[结论]成功地探索到赤霉素GA4+7在5000 L发酵罐中发酵工艺条件,GA4+7在5000 L发酵罐中发酵效价达到788 mg/L,且GA4/GA7比例为1.06,GA4+7产品质量好,该工艺条件为大罐发酵生产赤霉素GA4+7奠定了较坚实的基础。
[Aims] It was to find out the fermentation technology conditions for gibberellin GA4＋7 in 5000L tank. [Methods] The gibberellin GA4＋7 fermentation medium formulation and optimization of fermentation conditions for screening were studied in shake flask conditions and 5 000L tank respectively based on the multi-factor different levels of orthogonal experimental design. Gibberellin GA4＋7 fermentation units were detected by means of HPLC. [Results] Gibberellin GA4＋7 fermentation medium optimization formula obtained： starch ＋ glucose as 9.0%＋1.0%, peanut powder ＋ soybean powder as 1.2%＋0.6%, potassium dihydrogen phosphate and ammonium sulfate were 0.2% and 0.01% respectively. Optimized fermentation technology conditions for GA4＋7 in 5 000 L tank obtained： fermentation temperature as 32 ℃, fermentation ventilation （Vair ： Vbroth） as 0.4 ： 1, shake flask inoculation liquid for seed tank as 400 mL, ammonium sulfate content in seed tank culture medium as 0.05%, added sugar fermentation, seed tank inoculum age as 38 h, fermentation transplanting amount as 12%, and calcium carbonate dosage in fermentor tank culture medium as 0.2%. [Conclusions] The fermentation technology conditions for gibberellin GA4＋7 in 5000 L tank was obtained successfully, the fermentation titre of GA4＋7 in the 5000 L tank reached 788 mg/L with the ratio of 1.06 between GA, and GA7 Which showed GA4＋7 was of good quality, moreover, the process conditions found out yet laid solid foundation for the large tank fermentation of gibberellin GA4 ＋7.
出处
《农药》
CAS
北大核心
2011年第11期805-807,847,共4页
Agrochemicals
基金
江西省重点新产品开发项目(省科技厅200610719500
省经贸委2006-0103)
