摘要
目的探讨补肾化瘀方对载脂蛋白E基因缺陷(ApoE-/-)小鼠头臂干动脉粥样硬化斑块稳定性和斑块内细胞凋亡水平的影响及可能机制。方法 24只8周龄雄性ApoE-/-小鼠饲以"西方类型"膳食(高脂饲料)20周后,头臂干已经形成易损动脉粥样硬化(AS)斑块,随机将小鼠分为3组:补肾化瘀组、他汀组和盐水组,连续给药12周后处死动物,将头臂干动脉连续石蜡切片、HE染色后,观察小鼠动脉粥样斑块的形态,并用TUNEL法检测斑块内细胞凋亡水平。结果补肾化瘀方干预后动脉粥样斑块的校正脂质核面积(LCA/PA)减小,斑块纤维帽增厚,斑块趋于稳定;补肾化瘀组斑块中细胞凋亡指数(AI)显著降低(P<0.05),斑块内Bcl-2蛋白表达升高、Bax蛋白表达显著降低(P<0.01)。结论补肾化瘀方可不同程度地减小脂质核面积,增加纤维帽厚度,抑制斑块内细胞凋亡,起到稳定AS斑块的作用。
Objective To investigate the influences and possible mechanism of Bushen Huayu Fang on the stability of artherosclerosis plaque （ASP） in the artery of brachiocephalic trunk and apoptosis in plaque in mice with gene defect of apolipoprotein E （ApoE-/-）. Methods Male mice of ApoE-/- （8 weeks old, n =24） were fed with Western style diet （high-fat diet） for 20 weeks until the vulnerable ASP generated in the artery of brachiocephalic trunk. All mice were randomly divided into 3 groups： Bushen Huayu Fang group （Bushen group）, simvastatin group and model group. After continuous administration of medicinal for 12 weeks, the mice were sacrificed and the artery of brachiocephalic trunk was treated with paraffin sections and HE staining continuously for observing the histomorphological changes of ASP. The apoptosis in artherosclerosis plaque was detected by using TUNEL assay. Results After the intervention of Bushen Huayu Fang the corrected lipid core area （LCA/PA） of ASP was reduced, fibrous cap was thickened, and ASP trended to stable. In the Bushen group, the apoptosis index of ASP decreased significantly （P 〈 0.05）, the expression of Bcl-2 protein increasedand expression of Bax protein decreased significantly （P 〈 0. 01 ）. Conclusion Bushen Huayu Fang can reduce the lipid core area, thicken fibrous cap, inhibit apoptosis in the plaque and improve ASP stability in varying degrees.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第10期686-689,I0001,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
上海市浦东新区卫生局卫生科技青年基金资助项目(No.PW2010B-11)
