摘要
目的观察大鼠脑缺血再灌注早期梗死灶边缘区皮层Toll样受体9(Toll-like receptor 9,TLR9)信号通路的活化情况。方法制备Sprague-Dawley(SD)大鼠短暂大脑中动脉闭塞模型,缺血90 min后再灌注,随机分两组:6 h组(n=5)和3 d组(n=5),分别采用RT-PCR、Western-blot法测定梗死灶边缘区皮层TLR9、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、干扰素调节因子7(interferon regulatory factor 7,IRF7)、干扰素-β(interferon-beta,IFN-β)的mRNA及蛋白表达。结果 6 h和3 d组TLR9、TNF-α、IRF7、IFN-β的mRNA表达量分别为(0.43±0.03)和(0.93±0.03)、(1.21±0.11)和(2.26±0.16)、(0.44±0.04)和(1.27±0.17)、(0.15±0.02)和(0.26±0.03),蛋白表达量分别为(0.75±0.04)和(1.35±0.04)、(0.93±0.04)和(1.05±0.02)、(0.54±0.03)和(0.73±0.02)、(0.82±0.02)和(0.93±0.03)(组间比较,均P<0.01)。同组内TNF-α的表达明显高于IFN-β的表达(均P<0.01)。结论 TLR9信号通路在脑梗死早期的炎症反应中可能起重要作用。
Objective To study the activation of toll-like receptor 9 （TLR9） signaling pathway in the peri-in- farct cortex at the early stage of ischemia-reperfusion in a rat model of middle cerebral artery occlusion （MCAO）. Meth- ods The MCAO model was induced in male rats by using intraluminal filament technique and reperfusion was performed by removing the filament at 90 minutes after occlusion. The MCAO rats were randomly assigned to 2 groups：6 h group （n = 5） and 3d group （n = 5）. RT-PCR and Western blot were used to detect the mRNA and protein expression of TLR9, TNF-α, IRF7 and IFN-β in the peri-infarct cortex. Results The mRNA and protein expression of TLR9, TNF-α, IRF7 and IFN-βwere significantly higher in the 3 d group than in the 6 h group （mRNA：0.93± 0.03 vs. 0.43±0.03 on TLR9, 2.26±0.16 vs. 1.21 ±0.11 on TNF-α 1.27 ± 0.17 vs. 0.44 ± 0.04 on IRF7, 0.26 ± 0.03 vs. 0.15 ± 0.02 on IFN-β; protein： 1.35 ± 0.04 vs. 0.75 ± 0.04 on TLR9, 1.05± 0.02 vs. 0.93± 0.04 on TNF-α, 0.73 ± 0.02 vs. 0.54 ± 0.03 on IRF7, 0.93 ± 0.03 vs. 0.82 ± 0.02 on IFN-β; P 〈 0.01）. The mRNA and protein expression of TNF-α were significantly higher than IFN-β in the peri-itffarct cortex at all time points （P 〈 0.01 ）. Conclusions TLR9 signaling pathway may play a critical role in the inflammatory response at the early stage of cerebral infarction.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2011年第10期596-599,共4页
Chinese Journal of Nervous and Mental Diseases
基金
国家自然科学基金(编号:81171103)
广东省自然科学基金(编号:9151008901000028
S2011010006043)
