摘要
目的:探讨鸦胆子油乳在体外对人大细胞肺癌NCI-H460细胞的抑制作用及其机制。方法:以不同质量浓度鸦胆子油乳(2.5、5.0、10、20、40、80μg/ml)处理NCI-H460细胞,24、48、72h后收集细胞,MTT法检测鸦胆子油乳对NCI-H460细胞增殖的抑制作用;吉姆萨染色后光学显微镜下观察NCI-H460细胞形态学改变;流式细胞仪测定NCI-H460细胞凋亡率;caspase-3酶活性试剂盒检测NCI-H460细胞caspase-3酶活性。结果:鸦胆子油乳可剂量和时间依赖性抑制NCI-H460细胞的增殖,80μg/ml鸦胆子油乳作用72h时的抑制率达(91.07±1.60)%。鸦胆子油乳作用后,NCI-H460细胞呈现典型凋亡形态学改变;流式细胞术检测结果显示,10、20和40μg/ml鸦胆子油乳作用48h后,NCI-H460细胞凋亡率分别为(45.23±4.30)%、(54.14±3.09)%和(61.57±7.28)%(P<0.01)。鸦胆子油乳可剂量依赖性上调NCI-H460细胞caspase-3酶活性,40μg/ml鸦胆子油乳时caspase-3酶活性为(1.07±0.07)μmol/μg,是对照组的4.97倍(P<0.01)。结论:鸦胆子油乳可能通过活化caspase-3诱导NCI-H460细胞凋亡,抑制NCI-H460细胞增殖。
Objective：To investigate the inhibitory effect of Brucea javanica oil emulsion on the growth of human large-cell lung cancer NCI-H460 cells in vitro and its mechanisms.Methods：NCI-H460 cells were treated with different concentration of Brucea javanica oil emulsion（2.5,5.0,10,20,40 and 80 μg/ml） for 24,48,and 72 h.The inhibitory effects of Brucea javanica oil emulsion on proliferation of NCI-H460 cells was examined by MTT;morphological changes was observed by optical microscope after Giemsa staining;apoptotic rate of NCI-H460 cells was examined by flow cytometry;and caspase-3 activity in NCI-H460 cells was detected with caspase-3 activity kit.Results：Brucea javanica oil emulsion significantly inhibited the proliferation of NCI-H460 in a dose-and time-dependent manner,and the inhibiting rate reached（91.07±1.60）% after 80 μg/ml Brucea javanica oil emulsion treatment for 72 h.Typical apoptotic morphological changes also appeared after Brucea javanica oil emulsion treatment.Flow cytometry results demonstrated that the apoptotic rates of NCI-H460 cells were（45.23±4.30）%,（54.14±3.09）% and（61.57±7.28）% after 10,20 and 40 μg/ml Brucea javanica oil emulsion treatment for 48 h,respectively（P0.01）.In NCI-H460 cells,the Brucea javanica oil emulsion increased the activity of caspase-3 in a dose-dependent manner.after 40 μg/ml Brucea javanica oil emulsion treatment,caspase 3 activity reached 1.07±0.07 μmol/μg,which was 4.97 fold higher than control group（P0.01）.Conclusion：Brucea javanica oil emulsion can induce apoptosis and inhibit the proliferation of NCI-H460 cells,possibly through increasing caspase-3 activity.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2011年第5期519-523,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.30670916)
沈阳市科技局科技计划资助项目(No.1063315-1-00)~~
