摘要
目的:比较海南省汉族和黎族妇女亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C及甲硫氨酸合成酶还原酶(MTRR)A66G多态性分布情况,获取其多态性位点的群体遗传学特征。方法:采用横断面调查研究方法,以海南省内11437例汉族与黎族孕妇为对象,采集口腔黏膜上皮细胞,提取DNA,采用Taqman-MGB技术,进行编码基因MTHFR和MTRR的相关多态性位点检测。结果:汉族妇女MTHFR C677T位点基因型纯合突变TT型高于黎族妇女,黎族妇女MTHFRA1298C基因型纯合突变CC型、MTRR A66G基因型纯合突变GG型的频率高于汉族妇女(P<0.01)。结论:海南省汉族与黎族妇女MTHFR和MTRR基因多态性分布存在差异。
Objective： To explore the methylenetetrahydrofolate reductase （MTHFR） C677T, A1298C and methionine synthase reductase （MTRR） A66G gene polymorphisms among the Han and Li women in Hainan Province, and discuss the genetic characteristic of these two population. Methods ： A cross - sectional survey research method was used. A total of 11 437 Han and Li pregnant women were recruited and collected oral epithelial cells. The detections of MTHFR and MTRR gene polymorphisms were conducted with Taqman - MGB technology. Results ： The frequency of the MTHFR C677T TT genotype in the Han women was higher than that in women of Li ethnic minority. The frequencies of MTHFR A1298C CC genotype and MTRR A66G GG genotype in women of Li ethnic minority were higher than those of the Han women （ P all 〈 0.01 ）. Conclusion： There are differences in MTHFR and MTRR gene polymorphisms between the Han and Li women in Hainan Province.
出处
《中国计划生育学杂志》
北大核心
2011年第11期655-657,共3页
Chinese Journal of Family Planning
基金
海南省卫生厅科研项目(琼卫重点2010-44
琼卫2010-27)
