摘要
AIM To investigate the effect of Boschniakiarossica（BR）extract on expression of GST-P,p53 and p21<sup>ras</sup>proteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase（GST-P）,p53 and p21<sup>ras</sup>proteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H<sub>2</sub>O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver（GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P【0.05）andthe expression of mutant p53 and p21<sup>ras</sup>proteinwas lower than that of hepatic preneoplasticlesions（33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup）.Both CH<sub>2</sub>Cl<sub>2</sub> and H<sub>2</sub>O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema（inhibitory rateswere 26%-29% and 35%-59%,respectively）. BR-H<sub>2</sub>O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH<sub>2</sub>CI<sub>2</sub> and H<sub>2</sub>O extracts from BR exerted anti-inflammatory effect in rats and mice.
AIM To investigate the effect of Boschniakia rossica(BR)extract on expression of GST-P, p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis in rats and its anti- inflammatory activities. METHODS The expression of tumor marker- placental form glutathione S-transferase(GST- P),p53 and p21^(ras)proteins were investigated by immunohistochemical techniques and ABC method.Anti-inflammatory activities of BR were studied by xylene and croton oil-induced mouse ear edema,carrageenin,histamine and hot scald-induced rat pow edema,adjuvant-induced rat arthritis and cotton pellet-induced mouse granuloma formation methods. RESULTS The 500 mg/kg of BR-H_2O extract fractionated from BR-Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver(GST-P staining was 78% positive in DEN+AAF group vs 20% positive in DEN+AAF+BR group,P<0.05)and the expression of mutant p53 and p21^(ras)protein was lower than that of hepatic preneoplastic lesions(33% and 22% positive respectively in DEN+AAF group vs negative in DEN+AAF+BR group).Both CH_2Cl_2 and H_2O extracts from BR had anti-inflamatory effect in xylene and croton oil-induced mouse ear edema(inhibitory rates were 26%-29% and 35%-59%,respectively). BR-H_2O extract exhibited inhibitory effect in carrageenin,histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet-induced granuloma formation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH_2CI_2 and H_2O extracts from BR exerted anti-inflammatory effect in rats and mice.
基金
the National Natural Science Foundation of China,No.39660021
