摘要
Objective To investigate the special effect of donor antigen trichosnthin (TCS) conjugate on depleting the immune reactive cells that respond to xenograft, and thus inducing specific xenograft tolerance. Methods Rat anti mouse antibodies coupled sepharose 4B beads were used to purify mouse H 2 antigens by affinity chromatography. Those purified H 2 antigens were conjugated with TCS by heterobifunctional reagents SPDP [3 (2 pyridyldithio) propionic acid hydroxysuccinimide ester] and 2 IT (2 iminothiolane). The specific inhibition function of Ag TCS to recipient's immune cells was measured in vitro. Ag TCS conjugate (200 μg/kg, iv) was administered to the recipients (rats) 4 days prior to cardiac xenotransplantation of mouse to rat. Results In vitro, the proliferation of immune reactive cells in the recipients pretreated with Ag TCS was inhibited. The Ag TCS conjugate significantly prolonged the cardiac survival time (6.88±1.36 days) compared with cyclosporine A (CsA) group (2.83±0.75 days) (P<0.01). Conclusion Donor Ag TCS conjugate can induce specific tolerance to xenograft.
Objective To investigate the special effect of donor antigen trichosnthin (TCS) conjugate on depleting the immune reactive cells that respond to xenograft, and thus inducing specific xenograft tolerance. Methods Rat anti mouse antibodies coupled sepharose 4B beads were used to purify mouse H 2 antigens by affinity chromatography. Those purified H 2 antigens were conjugated with TCS by heterobifunctional reagents SPDP [3 (2 pyridyldithio) propionic acid hydroxysuccinimide ester] and 2 IT (2 iminothiolane). The specific inhibition function of Ag TCS to recipient's immune cells was measured in vitro. Ag TCS conjugate (200 μg/kg, iv) was administered to the recipients (rats) 4 days prior to cardiac xenotransplantation of mouse to rat. Results In vitro, the proliferation of immune reactive cells in the recipients pretreated with Ag TCS was inhibited. The Ag TCS conjugate significantly prolonged the cardiac survival time (6.88±1.36 days) compared with cyclosporine A (CsA) group (2.83±0.75 days) (P<0.01). Conclusion Donor Ag TCS conjugate can induce specific tolerance to xenograft.
