摘要
以肝吸虫成虫抗原进行Dot-ELISA试验,40例粪查阳性的肝吸虫病患者血清,有33(82.5%)例呈阳性反应。40例正常人中,有1例呈弱阳性反应。与血吸虫、布氏姜片虫、肺吸虫等血清均无交叉反应,显示有较好的特异性。用肝吸虫病患者阳性参考血清作不同稀释度的观察,其终点滴度为1:2560,表明有较高的敏感性。结果还表明,本法检测效果与常规ELISA相似,但Dot-ELISA更简便、经济,适于基层推广应用。
Dol-ELISA was preliminarily used in the diagnosis of Clonorchiasis sinensis.Among 40 confirmed Clonorchiasis sinensis cases 82.5% showed positive reac-tions, one serum of 40 sera from healthy adults was found rositive. No crossreaction was observed with sera from cases of Schistosomiasis, fasciolopsiasisand paragonimiasis and so on. The positive rate by Dot-ELISA was comparedwith that by ELISA and no significant difference was noted between them.This test appears to be sensitive specific for serodiagnosis of Clonorchiasissinensis.It is inexpensive and simple to perform. Minute volumes of antigen isrequired. It is recommended as a serological test of choice for the diagnosis ofClonorchiasis sinensis
出处
《中国医科大学学报》
CAS
CSCD
1991年第S1期26-28,共3页
Journal of China Medical University
