摘要
目的探讨应用高压氧治疗大鼠脑缺血再灌注损伤后,生长相关蛋白-43(GAP-43)在神经系统的表达。方法成年健康雄性SD大鼠32只,随机分为高压氧组(n=16)和高压空气组(n=16),每组再分为脑缺血再灌注模型组(n=8)和假手术组(n=8)。应用线栓法制备大鼠脑缺血再灌注动物模型,免疫组化法检测GAP-43在神经系统中的表达情况。结果应用高压氧治疗大鼠脑缺血再灌注损伤后,模型组GAP-43表达高于假手术组(P<0.05);但应用高压空气治疗后,模型组GAP-43表达略高于假手术组,差异无统计学意义(P>0.05)。高压氧治疗模型组GAP-43表达高于高压空气治疗模型组,差异有统计学意义(P<0.05)。结论应用高压氧治疗能够增强GAP-43的表达,有利于受损神经系统的结构修复和功能恢复。
Objective To investigate the effect of hyperbaric oxygen therapy on the expression of growth associated protein-43 （ GAP-43 ） in the nervous system after cerebral ischemia-reperfusion injury in rats. Methods 32 male adult Sprague-Dawley（SD） rats were randomly divided into hyperbaric oxygen group （n=16） and high pressure air group （n=16）. Each group was subdivided into two subgroups： model group （n=8） and sham operation group （n=8）. The animal model of cerebral ischemia-reperfusion was established in rats by suture method. Expression of GAP-43 in the nervous system was detected by immunohistochemistry. Results With the application of hyperbaric oxygen, the expression of GAP-43 in model group was higher than that in sham operation group （P〈0.05）. But in high pressure air group there was no significant difference between two subgroups （P〉0.05）. The expression of GAP-43 in model group of hyperbaric oxygen was higher than that in model group of high pressure air （P〈0.05）. Conclusion Hyperbaric oxygen therapy can enhance expression of GAP-43 and repair the structure and function of the damaged nervous system.
出处
《分子诊断与治疗杂志》
2011年第6期388-390,共3页
Journal of Molecular Diagnostics and Therapy
关键词
脑缺血
生长相关蛋白-43
高压氧
神经系统
Cerebral ischemia
Growth associated protein-43
Hyperbaric oxygen
Nervous system
