摘要
利用胰蛋白酶消化法成功启动了松江鲈鳍细胞的原代培养,并通过利用不同培养基、培养温度、pH值对松江鲈鳍细胞进行体外培养实验。实验结果显示:含20%FBS的DMEM/F12(pH=7.2)是松江鲈鳍细胞体外培养的最适培养基,鳍细胞的最适培养温度为24℃。
The primary culture of Trachidermus fasciatus fin cells was successfully launched by trypsin digestion.Furthermore,Trachidermus fasciatus fin cells were cultured in vitro with various culture medium and pH values at different temperatures.The results showed that DMEM / F12（pH = 7.2） containing 20% FBS was the optimal medium for Trachidermus fasciatus fin cells cultivation in vitro and the optimal culture temperature was 24 ℃.
出处
《辽东学院学报（自然科学版）》
CAS
2011年第3期194-197,共4页
Journal of Eastern Liaoning University:Natural Science Edition
基金
辽东学院自然科学基金项目(2006-Z04)
关键词
松江鲈
鳍细胞
体外培养
Trachidermus fasciatus
fin cell
cultivation in vitro
trypsin digestion
