摘要
在以密阳46为母本的杂交后代中发现不育材料H236A,通过杂交和自交,确定其不育类型和不育度;通过碘染和徕卡荧光显微镜DM2500对成熟花粉粒观察,确定花粉粒育性、败育形态和时期。结果表明:H236A是胞质雄性不育,不育度达99.8%以上;花粉粒属典败型的达83.17%,圆败型占16.83%,没有染败型花粉粒,为单核期败育。花粉粒败育形态多种多样,有不规则形、梭形、圆形等。清晰观察到晚期小孢子细胞质定向移动形成细胞质桥现象。本文还讨论了成熟花粉粒败育时期和败育形态的划分。
Diversity of cytoplasmic male sterility is key measure for solving grain quality,resistance and yield of three line hybrids.Sterile plants H236A were found in offspring from Miyang 46 as female parent.Its sterile type and abortion degree were determined by hybridization and selfing.Its pollen grain fertility,abortion shape,and abortion stage were studied by 1%I2-KI staining and observation of Leica fluorescence microscope DM 2500 on mature pollens.Results showed that H236A was cytoplasmic male sterility,which abortive degree was above 99.8%.Pollen grains belong to typical abortive was 83.17% and round abortive was 16.83%,no stained abortive pollen grains.Abortive stage of H236A was in single nuclear development period.Abortive shapes of pollen grains were in varied forms,such as irregular,round and spindle etc.Phenomenon that cytoplasm of late microspore moved directionally and formed cytoplasm bridge was observed clearly.Division of mature pollens abortive stage and shape were also discussed in this paper.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第6期1014-1018,共5页
Journal of Plant Genetic Resources
基金
四川省教育厅重点项目(07ZA130)
转基因生物新品种培育重大专项(2008ZX08001-001)
西南科技大学实验技术重点项目(09syjs-03)
