摘要
目的了解2006年1月-2011年8月期间中山地区无偿献血者梅毒感染状况，评价梅毒筛查试剂盒质量，为保证血液安全提供有效的预防措施。方法用ELISA试剂检测献血者梅毒螺旋体（TP）抗体，阳性者再用TP明胶凝集试验（TPPA）确认，分析梅毒感染率者的流行病学特征。结果共检测232484名无偿献血者TP抗体，阳性1092例（占0．47％）；TP阳性率，在年度间、男女性别、不同采血点、个体与集体无偿献血者间的差别无意义，雅均〉0．05，但不同年龄组差异有极显著性，P〈0．005；初、复检ELISA检测灵敏度已达到99．42％和99．65％，但特异性只有27．81％和63．91％，产生一定的假阳性结果。结论中山地区无偿献血人群梅毒感染率保持相对稳定水平，无明显上升趋势；对献血者ELISA中TP阳性标本用TPPA确认非常有意义；尽量输注在4℃保存3天以上的血液。
Objective To explore the current situation of syphilis infection in voluntary blood donors in Zhongshan, to evaluate the quality of syphilis screening kit and to provide effective preventive measures for blood safety. Methods Treponemal antibody was detected by ELISA in all the Blood donors and those with antibody positive and then comfirmed by TP particle agglutination test （ TPPA ）. The epidemiological characteristics of syphilis infection were analyzed. Results During the period of January 2006 to June 2011, a total of 232,484 blood donors were detected for treponemal antibody, 1092 of whom （ 0.47% ） were positive. The positive rate of TP did not differ significantly between years, male and female, different blood collection spots, individual and group donors （ P〉 0.05 ）, but differed significantly among different age groups （P〈 0.005 ）. The sensitivity of initial ELISA was 99.42% and that of repeat ELISA was 99.65%, but the specificity was only 27.$1% and 63.91%, respectively. Some false positive results were found. Conclusions The rate of syphilis infection in voluntary blood donors in Zhongshan area during January 2006.1 to June 2011.6 remains at a relatively stable level. It is of significance to have TPPA confirm the positive samples detected by ELISA in blood donors.
出处
《国际医药卫生导报》
2011年第23期2956-2960,共5页
International Medicine and Health Guidance News
关键词
献血者
梅毒螺旋体
酶联免疫吸附试验
梅毒螺旋体明胶凝集试验
Blood donor
Treponema pallidum
Enzyme linked immunosorbent assay
Treponema pallidum particle agglutination test
