摘要
目的对850例疑似人乳头瘤病毒(HPV)感染的女性进行HPV基因分型检测,以探讨其临床意义。方法用专用宫颈刷采集妇女宫颈的脱落细胞,采用基因芯片法检测850例患者HPV临床样本,并进行基因分型。结果 850例样本中,HPV感染325例,阳性率38.2%。共检出18种HPV亚型,检出感染单一HPV亚型177例(20.8%),感染两种亚型以上148例(17.4%)。单一型别阳性中低危型主要为HPV 11、42、CP8034、44,高危型主要为HPV 16、18、31、33、45、52、53、56等。结论基因芯片技术可一次检测多种亚型,有利于HPV多重感染的诊断和宫颈癌的防治,可作为宫颈癌筛查的手段。
Objective To detect the genotypes of human papillomavirus infection（HPV） in 850 women who were suspected to have HPV infection,and to investigate its clinical significance.Methods Cervical cast-off cells were acquired with the special cervix brush.DNA chip technique was applied to detect HPV genotypes in 850 clinical specimens.Results Totally 325 cases（38.2%） were HPV positive among 850 clinical specimens.A total of 18 subtypes were detected.In 325 cases of HPV infection,177 cases（20.8%） were infected with single HPV subtype,while 148 cases（17.4%） were infected with two or more HPV subtypes.HPV11,42,CP8034,44 were the most frequent low-risk subtypes,and in the high-risk subtypes,HPV16,33,52,58,18 were dominant.Conclusion Gene chip technique is useful for the diagnosis of muti-HPV infection and therefore can be used for the prevention and treatment of cervical cancer.
出处
《检验医学与临床》
CAS
2011年第23期2858-2859,2861,共3页
Laboratory Medicine and Clinic
关键词
人乳头瘤病毒
宫颈癌
基因芯片技术
基因型
human papillomavirus
cervical cancer
gene chip technique
genotype
