摘要
目的探讨银杏达莫注射液对2型糖尿病肾病(DN)大鼠肾脏转化生长因子(TGF)-β1表达的影响,从而探讨银杏达莫注射液对DN的治疗作用。方法选取实验大鼠60只,以高热量饲料加小剂量链脲佐菌素(STZ)建立DN大鼠模型,将大鼠随机分为正常组、DN组和治疗组(每组各10只),观察各组大鼠一般情况及24 h蛋白尿、血糖、肾脏病理改变;采用免疫组化法检测TGF-β1的表达。结果糖尿病组表现为大量蛋白尿、肾组织病理损害、TGF-β1高表达,与正常组有显著差异;治疗组24 h尿蛋白与正常组相比显著增高,与DN组相比有显著降低,肾组织病理损害明显减轻,TGF-β1表达高于正常组,低于ND组,差异显著。结论银杏达莫注射液可以通过降低TGF-β1表达对2型糖尿病DN大鼠肾脏起到保护作用,延缓DN发展。
Objective To investigate the effect of ginkgo-dipyridamolum（GLEDI） on type 2 diabetes nephropathy rats（2-DN） and the expression of TGF-β1 in 2-DN rats renal.Methods The Wistar rats were randomly divided into diabetic model,diabetic model treated with GLEDI and normal control groups.At the end of 12 weeks,the protein expression and secretion of TGF-β1 was determined by immunohistochemistry,Furthermore blood glucose,blood lipid,blood urea nitrogen（BUN） and blood creatinine（SCr） were measured.Results The tratio of renal weight / body weight,and the level of BUN and Scr of diabetic model group and GLEDI treated group were significantly higher than those of normal control group（P〈0.01）.The tratio of renal weight / body weight and the level of BUN and Scr of GLEDI treated group were siginificantly lower than those of diabetic model group（P〈0.01）.The expression of TGF-β1 protein in diabetic model group and GLEDI treated group were siginificantly higher than that of normal control group,and TGF-β1 protein in treatment group was lower than that of diabetic model group.Conclusions Ginkgo-dipyridamolum has renal protective effect on 2 DN rats by reducing the expression of TGF-β1 of renal tissue.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2011年第23期4604-4606,共3页
Chinese Journal of Gerontology
基金
黑龙江省卫生厅资助项目(No.2010-289)
黑龙江省研究生创新科研资金项目(YJSCX2011-293HLJ)
