摘要
目的探讨丙型肝炎患者保护性免疫缺陷的原因。方法根据HCVC区和NS4区基因序列设计并人工合成3条合成肽,采用抗原捕捉酶联免疫吸附试验检测HCV感染者血清中抗-HCV IgG抗体轻链κ/λ比值,同时以正常人血清做对照。结果抗-HCV SP42,CP10和CP9抗体轻链的表达呈明显的偏斜;116例抗-HCV阳性者中113例(97.41％),抗-HCV IgG抗体轻链κ/λ比值偏高,所有病例追踪观察1a(其中11例抗-HCV阳性者随访2a);30例患者接受α-干扰素治疗,发现抗-HCV抗体κ/λ比值恒定不变。结论 HCV感染者抗-HCV抗体的产生不均匀性并呈稳定的克隆性。B细胞克隆优势化可能是HCV感染后机体保护性免疫缺陷的原因之一。
AIM To study the cause of protective immunodeficiency of patients with hepatitis C.METHODS An antigen capture ELISA in which HCV synthetic peptides SP42, CP10 and CP9 were derived from HCV NS4 and core gene region, respectively, was used as solid-phase antigens to detect the differences in light chain isotype expression of anti-HCV antibodies. RESULTS Antibodies in 116 sera of HCV-infected patients against HCV SP42, CP10 and CP9 were characterized by a skewed light chain isotype expression. One hundred and thirteen out of 116 sera samples of HCV infection (97.41%) showed at least one of the three anti-HCV antibodies skewed from the normal ratio of light chain isotype kappa/lambda. The kappa/lambda ratios of anti-HCV in patients with hepatitis C were found to be unique and constant in all for one year follow-up. CONCLUSION Anti-HCV response was stable and clonally restricted in HCV infection. B-cell clonal dominance may be the cause of human protective immunodeficiency after HCV infection.
出处
《世界华人消化杂志》
CAS
2000年第1期28-30,共3页
World Chinese Journal of Digestology
关键词
丙型肝炎
免疫学
HCV
免疫缺陷
hepatitis C/ immunology
hepatitis C virus
immunodeficiency
