摘要
采用分光光度法,以邻硝基苯基荧光酮(O-NPF)为显色剂,研究了O-NPF与头孢唑啉(CFZ)络合物的可见光谱性质及测定条件。结果表明:CFZ在4.545～72.72μg/mL范围内遵循比尔定律,回归方程为:A=0.668ρ+0.5341,检出限1.53μg/mL,表观摩尔吸光系数为5.41×104L.mol-1.cm-1,相关系数为R2=0.9891,并探讨了其反应机理。方法已用于尿样及生物样品中CFZ含量的测定。
The visible spectral characterist ic and the reaction condition of the complex of o-nitro phenyl fluorone（O-NPF） and cefazolin（CFZ） were investigated by spectrophotometry with O-NPF as a developer.The results showed that the concentration of CFZ obeyed the Beer＇s law in the range of 4.545～72.72μg /mL and the regression equation was A=0.668ρ＋0.5341 with the correlation coefficient r of 0.9891,the apparent molar absorptivity was 5.41×104 L·moL-1·cm-1and the detection limit was 1.53μg /mL.Further more,the reaction mechanism had been explored.The proposed method has been successfully applied to the determination of CFZ in urine and biological samples,and the results were satisfying.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第1期102-104,共3页
Chinese Journal of Analysis Laboratory
基金
内蒙古自然科学基金项目(2009MS0206)
内蒙古大学创新人才培养项目(2-1
2.1-042)资助
