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百脉根离子通道蛋白Pollux与翻译延伸因子EF1A的相互作用 被引量：4

Interaction between ion channel protein Pollux and elongation factor 1A of translation in Lotus japonicus
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摘要 利用酵母双杂交技术,以百脉根离子通道蛋白Pollux胞内结构域为诱饵,从百脉根AD-cDNA文库中筛选到与Pollux相互作用的多个阳性克隆,经DNA序列测定及NCBI数据库BLAST分析,其中有6个阳性克隆编码翻译延伸因子EF1A。进一步在大肠杆菌中表达与纯化Pollux和EF1A融合蛋白,通过Pull-down及Western杂交证实Pollux胞内结构域与EF1A的C-末端相互作用。 Using ion channel protein Pollux-C containing RCK domain as a bait, 14 yeast colonies were screened from Lotus japonicus AD-cDNA library with yeast two-hybrid technique. Six positive clones were found to encode elongation factor 1A （EF1A） of translation through DNA sequencing and BLAST against NCBI database. The fusion tag protein of Pollux-C and EF1A expressed in E. coli were purified. The intra-cellular domain of Pollux interacted with the C-terminus of EF1A was verified by pull-down and western blotting in vitro. The results might lay a foundation for further researches on the regulating mechanism and biological function of EF1A in symbiosis signaling in legume.
作者 代广中 袁兆栋 苟洪兰 方庆 朱辉 张忠明
机构地区 华中农业大学生命科学技术学院/农业微生物学国家重点实验室
出处 《华中农业大学学报》 CAS CSCD 北大核心 2012年第1期34-38,共5页 Journal of Huazhong Agricultural University
基金 国家自然科学基金(30870186) 国家重点基础研究发展计划"973"课题(2010CB126502) 国家转基因重大专项课题(2009ZX08009-116B)
关键词 百脉根 离子通道蛋白 酵母双杂交 翻译延伸因子 Lotus japonicus ion channel protein yeast two-hybrid EF1A
分类号 S154.381 [农业科学—土壤学]
  • 相关文献

参考文献17

  • 1STRACKE S,KISTNER C, YOSHIDA S,et al. A plant receptor-like kinase required for both bacterial and fungal symbiosis [J]. Nature, 2002,417(6892) : 959-962.
  • 2RADUTOIU S, MADSEN L H, MADSEN E B, et al. Plant recognition bacterial requires two lysM receptor like protein kinases[J]. Nature, 2003,425 : 585-591.
  • 3OLDROYD G E D, DOWN1E J A. Coordinating nodule morphogenesis with rhizohial infection in legumes[J]. Annual Review of Plant Biology,2008,59(1):519-546.
  • 4IMAIZUMI-ANRAKU H, TAKEDA N, CHARPENTIER M, et al. Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots[J]. Nature, 2005,433(7025):527-531.
  • 5CHARPENTIER M, BREDEMEIER R, WANNER G, et al. Lotus japonicus CASTOR and POLLUX are ion channels essential for perinuclear calcium spiking in legume root endosymbiosis[J]. The Plant Cell, 2008,20 (12):3467-3479.
  • 6CHEN C, FAN C, GAO M, et al. Antiquity and function of Castor and Pollux, the twin ion channel-encoding genes key to the evolution of root symbioses in plants[J]. Plant Physiology, 2009,149 (1):306-317.
  • 7杨绮,朱辉,陈春芬,朱浩,张忠明.应用酵母双杂交系统筛选与CASTOR相互作用的蛋白[J].农业生物技术学报,2007,15(6):1012-1018. 被引量：2
  • 8储晓洁,康恒,胡晓晶,张忠明.百脉根结瘤信号通道蛋白NSP1和NSP2的转录激活作用[J].华中农业大学学报,2010,29(5):582-587. 被引量：4
  • 9胡晓晶,储晓洁,康恒,朱辉,张忠明.百脉根结瘤信号通道蛋白NSP1和NSP2的相互作用[J].华中农业大学学报,2011,30(2):132-137. 被引量：4
  • 10KANG H, ZHU H,CHU X, et al. A novel interaction between CCMK and a protein containing the Scythe_N ubiquitin-like domain in Lotus japonicus [J]. Plant Physiology, 2011, 155 (3) : 1312-1324.

二级参考文献79

  • 1谭汝芳,李玲.小G蛋白ROP的研究进展[J].生命科学研究,2006,10(S1):80-84. 被引量：4
  • 2胡国元,周俊初.华癸中生根瘤菌(Mesorhizobium huakuii)研究进展[J].华中农业大学学报,2006,25(2):213-218. 被引量：5
  • 3OLDROYD G E D,DOWNIE J A.Coordinating nodule morphogenesis with rhizobial infection in legumes[J].Annu Rev Plant Biol,2008,59:519-546.
  • 4MADSEN E B,MADSEN L H,RADUTOIU S,et al.A receptor kinase gene of the LysM type is involved in legume perception of rhizobial signals[J].Nature,2003,425(6958):637-640.
  • 5KISTNER C,WINZER T,PITZSCHKE A,et al.Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis[J].Plant Cell,2005,17(8):2217-2229.
  • 6KALO P,GLEASON C,EDWARDS A,et al.Nodulation signaling in legumes requires NSP2,a member of the GRAS family of transcriptional regulators[J].Science,2005,308(5729):1786-1789.
  • 7SMIT P,RAEDTS J,PORTYANKO V,et al.NSP1 of the GRAS protein family is essential for rhizobial Nod factor-induced transcription[J].Science,2005,308(5729):1789-1791.
  • 8OLDROYD G E D,LONG S R.Identification and characterization of nodulation-signaling pathway 2,a gene of Medicago truncatula involved in Nod factor signaling[J].Plant Physiology,2003,131(3):1027-1032.
  • 9TIRICHINE L,IMAIZUMI-ANRAKU H,YOSHIDA S,et al.Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development[J].Nature,2006,441(7097):1153-1156.
  • 10GLEASON C,CHAUDHURI S,YANG T,et al.Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition[J].Nature,2006,441(7097):1149-1152.

共引文献11

同被引文献55

  • 1孙伟,李燕,赵彦修,张慧.盐地碱蓬延伸因子 (Ss EF-1α)的克隆与表达分析(英文)[J].西北植物学报,2004,24(9):1657-1661. 被引量：10
  • 2KASHIWAGI T, YOKOYAMA K, ISHIKAWA K, et al. Crystal structure of microbial transglutaminase frorn Streptoverticilliuzn mobaraense[J]. Biol Chem,2002,277:44252-44260.
  • 3TANAKA Y,TSURUDA Y, NISHI M, et al. Exploring enzymatic catalysis at a solid surface a case study with transglutaminase-me- diated protein irnmobilization[J]. Org Biomol Chem, 2007,5:1764- 1770.
  • 4SMIDDY M A, MARTIN J, KELLY A L. Stability of casein micelles cross-linked by transglutami[J]. Journal of Dairy Sci- ence, 2006,89 : 1906-1914.
  • 5ABE H,CKYTO M, KAM1YA N. Protein lipidadon catalyzed by mi- crobial transglutaminase[J]. Chemistry, 2011,17 : 140004-14000K.
  • 6ANDO H, ADADI M, UMEDA K, et al. Purification and char- acterization of a novel transglutaminase derived from microor- ganism[J]. Biol Chem, 1989,53 : 2613-2617.
  • 7YU J Y,WU S C, HUNG H C, et al. Overproduction of soluble recombinant transglutaminase from Streptomyces netropsis in Escherichia coli [J]. Applied Microbiology and Biotechnology, 2008,81 : 523-532.
  • 8SOMMER C, VOLK N, PIETZSCH M. Model based optimiza- tion of the fed-batch production of a highly active transglutami- nase variant in Escherichia coli [J]. Protein Expr Purif, 2011, 77(1):9-19.
  • 9LIU X Q, YANG X Q, XIE F H, et al. On-column refolding and purification of transglutaminase from Streptomycesfradi- ae expressed as inclusion bodies in Escherichia coli[J]. Protein Expression and Purification, 2007,51 : 179-186.
  • 10DONOVAN R S, ROBINSON C W, GI.ICK B R. Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lae promoter[J]. Journal of India Microbiology, 1996,16 : 145-154.

引证文献4

二级引证文献12

华中农业大学学报
2012年 第1期

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