摘要
采用国内α 淀粉酶生产常用菌株BF7658变异菌种 ,直接以麸皮为原料固态发酵法生产α 淀粉酶 ,得到较适宜的条件为 :培养基初始含水量 (质量分数 )为 60 % ,起始 pH自然 ,液体接种量为 5mL/kg ,3 7～ 3 9℃培养 4 8～ 60h ,三角瓶培养产酶水平可达 1 2 4 8U/ g ,浅盘培养平均产酶可达 1754U/ g .固态发酵生产细菌α 淀粉酶产酶水平为液体深层发酵法的 4～ 5倍 ,并且成本低廉 ,具有较好的经济和环境效益 .
In this paper, solid state fermentation for production of alpha amylase was studied in a pure wheat bran medium. With the initial water content 60%,initial pH natural, incubation temperature 37～39 ℃,the liquid inoculum size 0.5%, cultivation in 250 mL Erlenmeyer flask yielded an enzyme titre of 1 248 U/g dry bacterial bran at 48～60 h. Under these above conditions, cultivation in trays yielded an average enzyme titre of 1 754 U/g,which was 4～5 times higher but cost less than in Submerged fermentation.
关键词
Α-淀粉酶
固态发酵
解淀粉芽孢杆菌
制备
alpha amylase
solid state fermentation
Bacillus amyloliquefaciens
