摘要
目的建立SKH-1无毛小鼠感染MRSA致菌血症模型的方法及其评价体系。方法 62只SPF级SKH-1无毛小鼠随机分成PBS对照组、低剂量模型组、高剂量模型组及给药组,尾静脉注射无菌PBS至对照组小鼠体内,其余各组小鼠尾静脉注射相应剂量MRSA(ST-239)菌悬液,从体重、死亡率、血常规、血液细菌浓度、主要器官荷菌量及病理改变等方面对模型进行评价,并用抗生素替考拉宁对模型进行检验。结果各组感染小鼠体重均下降明显,血常规指标改变明显;高剂量组死亡率高,血液中细菌浓度较高,肝、肾等多组织器官播散严重,皮肤可见多发性脓肿灶;使用替考拉宁能显著降低小鼠死亡率,减轻主要器官的损伤程度。结论应用MRSA菌株可成功诱导建立SKH-1无毛小鼠菌血症模型,模型具有病理特征明显及观察简便的优点,为相关药物评价研究提供了一个理想的动物模型。
Objective To establish a mouse model of bacteraemia infected with methicillin resistant Staphylococcus aureus（MRSA） and its evaluation system.Methods Sixty-two specific pathogen free SKH-1 hairless mice were randomly divided into 4 groups： the PBS,low-dose infection,high-dose infection and drug-treated infection groups.Mice were challenged with MRSA（ST-239） by intravenous tail injection.The bacteraemia models were evaluated by body weight,mortality,blood tests,blood bacterial concentrations,organs infection measuring and pathological examination,and the models were also verified with antibiotic teicoplanin.Results The infected mice had evident weight loss,high mortality,sharp changes in blood tests,high bacterial concentration in the blood and pathological alterations in many organs sush as in the liver and kidney.Teicoplanin reduced the mortality markedly and alleviated lesions of organs.Conclusions A SKH-1 hairless mouse model of bacteraemia has been successfully established by MRSA infection.This mouse model has apparent pathological features,is easy to be observed,and provides an ideal animal model for MRSA-related drugs evaluation studies.
出处
《中国实验动物学报》
CAS
CSCD
2011年第6期512-516,I0003,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
上海市科技发展基金项目(编号:09140900700)
