摘要
目的利用4种发根农杆菌15834,R1601,1025,1000诱导黄秋葵产生毛状根,建立黄秋葵的毛状根培养体系。方法利用共培养法研究不同外植体、菌株、预培养时间和侵染时间等对黄秋葵毛状根诱导率的影响和不同液体培养基对毛状根生长的影响筛选出最佳培养基,并研究了不同蔗糖浓度和外源激素对毛状根生长的影响。结果利用发根农杆菌R1601,预培养48 h的叶片为转化材料,感染8 min的诱导率最高,最佳培养基为MS液体培养基。结论黄秋葵毛状根离体培养体系的建立,为研究黄秋葵的有效药用成分的大规模生产奠定了基础。
Objective To induce Abelmoschus esculentus hairy roots by the infection of four kinds of Agrobacterium rhizogenes strains 15834,R1601,1025 and 1000 and establish an in vitro culture system of the hairy roots. Methods Hairy roots were induced by co-culture.Effects of explants,Agrobacterium rhizogenes,preculture time along with infecting time on the induction rate and effects of basic media,different sucrose concentration and exogenous phytohormones on growth of hairy root were studied. Results The highest induction rate was obtained from leaf with 48h preculture which were induced by R1601 for 8 min.The growth of hairy root could be raised by culture media with 0.5 mg/L IAA.Through the screen of basic media and the research of growth curve of hairy roots,the optimum one was MS medium. Conclusion Establishment of Abelmoschus esculentus hairy root culture can provide a foundation for the industrial production of active drug component.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第12期2991-2994,共4页
Lishizhen Medicine and Materia Medica Research
基金
中国博士后科学基金(No.20090461042)
关键词
黄秋葵
发根农杆菌
毛状根
Abelmoschus esculentus L.Moench
Agrobacterium rhizogenes
Hairy roots
