摘要
目的:探讨Rho激酶抑制剂法舒地尔对高血压大鼠勃起功能的影响及其机制。方法:12周龄雄性SD大鼠随机分成对照组(A组)、高血压组(B组)、法舒地尔治疗组(C组),建立高血压大鼠模型后,C组给予法舒地尔[30 mg/(kg.d)]腹腔注射,A组、B组给予等量生理盐水腹腔注射,术后10周测量大鼠阴茎海绵体内压/平均颈动脉压(ICPmax/MAP),Western印迹法测定ROCK1、ROCK2蛋白在阴茎海绵体的表达水平。结果:B组收缩压(mmHg)、ROCK1、ROCK2蛋白表达(190.39±5.07、0.048±0.002、0.143±0.011)较A组(124.81±4.01、0.036±0.001、0.101±0.011)显著增加(P<0.05),C组收缩压(mmHg)、ROCK1、ROCK2蛋白表达(182.03±4.32、0.044±0.001、0.126±0.007)较B组显著降低(P<0.05),B组ICPmax/MAP(36.82±5.47)较A组(59.99±5.69)显著降低(P<0.05),C组(51.1±5.63)较B组显著提高(P<0.05)。结论:法舒地尔可通过抑制RhoA/Rho激酶信号高表达及可能的降血压作用而改善高血压大鼠勃起功能。
Objective： To investigate the effect of fasudil,an inhibitor of Rho kinase,on the erectile function of hypertensive rats and its action mechanism.Methods： Twenty 12-week-old healthy male Sprague-Dawley rats were randomly divided into groups A（control）,B（hypertension） and C（fasudil treatment）.After establishment of the hypertension model,group C received intraperitoneal injection of fasudil at 30 mg/（kg·d）,while A and B normal saline only.At 10 weeks after surgery,we measured the corpus cavernosum pressure / mean carotid arterial pressure（ICPmax / MAP）,and the expression levels of ROCK1 and ROCK2 proteins in the corpus cavernosum of the rats by Western-blot.Results： The systolic blood pressure（mmHg） and the expressions of ROCK1 and ROCK2 proteins were significantly increased in group B（190.39±5.07,0.048±0.002 and 0.143±0.011） as compared with A（124.81±4.01,0.036±0.001 and 0.101±0.011）（P〈 0.05）,but markedly decreased in group C（182.03±4.32,0.044±0.001 and 0.126±0.007） in comparison with B（P〈 0.05）.ICPmax / MAP was significantly lower in group B（36.82±5.47） than in A（59.99±5.69）（P〈 0.05）,but remarkably higher in group C（51.1±5.63） than in B（P〈0.05）.Conclusion： Fasudil can improve erectile function in hypertensive rats by inhibiting the expression of RhoA / Rho kinase signaling and its possible attenuating effect on hypertension.
出处
《中华男科学杂志》
CAS
CSCD
2012年第1期11-15,共5页
National Journal of Andrology
基金
国家自然科学基金(81070486)
四川省杰出青年学术技术带头人培育项目(2010JQ0040)
四川省卫生厅科研基金(090214)~~
