摘要
目的观察兔附件扭转后卵巢组织的病理变化。方法建立兔附件扭转模型。40只日本大耳白兔采用随机数字法分为附件扭转(adnexal torsion,AT)模型组(n=32)和对照组(n=8)。模型组兔将左侧附件按顺时针方向扭转3周后固定于左侧腹壁,然后再将之分成4组,每组8只,分别于扭转24、48、72、96h后解除扭转并取双侧卵巢;对照组假手术96h后再次开腹取双侧卵巢。所有切除之右侧卵巢行病理学对照。结果左侧附件扭转24h后可见炎细胞浸润、卵巢细胞及卵细胞水肿;48h见较多的炎细胞浸润,颗粒细胞结构紊乱、水肿;72h见大量炎细胞浸润,颗粒细胞结构损坏和局灶性坏死,卵细胞水肿;96h见弥漫性颗粒细胞坏死及卵细胞坏死;卵巢组织病理评分(左侧vs右侧)呈现相同的时相性变化:24h(5.23±1.20)vs(0.65±0.51),P<0.05;48h(7.81±1.29)vs(0.59±0.42),P<0.01;72h(7.49±1.43)vs(0.67±0.61),P<0.01;96h(8.37±1.19)vs(0.73±0.61),P<0.01。AT组卵巢均有一氧化氮合成酶(iNOS)程度不等表达,随时间进展72h达高峰,96h下降。结论兔附件扭转后卵巢产生不同程度损伤,72h达不可逆性改变。
Objective To observe the pathological change in rabbit ovary after adnexal torsion. Methods A adnexal torsion （AT） model of rabbit was established. Forty Japanese long-ear white rabbits were randomly divided into AT model group（n=32） and control group（n=8）. The left adnexas of rabbits in AT model group were twisted 3 circuits in clockwise and fixed on the left abdominal wall. Rabbits in the AT model group were then divided into 4 subgroups（8 in each）. Bilateral ovaries in each subgroup were removed 24, 48, 72 and 96h after adnexal torsion, respectively. The bilateral ovaries in control group were removed 96h after sham operation. All the removed right ovaries were used as pathological internal-controls. Results Infiltration of a small number of inflammatory cells, ovarian cells, and edema of ovarian cells were observed 24h after torsion of the left adnexa. Infiltration of more inflammatory ceils, structure disorder and edema of granular ceils were found 48h after torsion of the left adnexa. Infiltration of a large number of inflammatory cells, structure disorder and focal necrosis of granular cells, and edema of ovarian cells were seen 72h after torsion of the left adnexa. Diffuse necrosis of granular and ovarian cells was found 96h after torsion of the left adnexa. The pathological score of the left and right ovarian tissues was 5.23 ± 1.20 and 0.65 ± 0.51, 7.81 ± 1.29 and 0.59 ± 0.42, 7.49 ± 1.43 and 0.67 ± 0.61, 8.37 ± 1.19 and 0.73 ± 0.61, respectively, 24, 48, 72 and 96h after torsion of the left and right adnexas. The expression level of iNOS was different in ovaries of the AT model group and reached its peak 72h and 96h after adnexal torsion. Conclusion Different extension injuries occur after adnexal torsion, which are reversible after 72h.
出处
《军医进修学院学报》
CAS
2012年第1期71-74,共4页
Academic Journal of Pla Postgraduate Medical School
基金
辽宁省自然科学基金项目(20102240)~~
关键词
兔
病理学
卵巢
扭转
rabbits
pathology
ovary
torsion
