摘要
目的 通过研究细胞骨架的变化 ,探讨脑梗死后神经元及暗神经元损伤的程度。方法7例尸检梗死脑做全脑大切片染色后 ,将梗死及其周围分成 4个区 (0～ 3区 ) ,各区取小块组织 ,用组织化学和免疫组织化学的方法观察梗死脑的暗神经元、微管相关蛋白 (MAP2 )、相对分子质量为2 0 0 0 0 0的神经丝 (NF2 0 0 )和星形细胞中的胶质纤维酸性蛋白 (GFAP) ,认识暗神经元、细胞骨架与梗死病理过程之间的关系 ,并进行病灶内外及其与对侧脑组织细胞骨架变化的对比。结果 1区暗神经元约为 90 % ;2区为 40 %～ 5 0 % ,有些暗细胞伴有细胞骨架的严重变化 ,有的细胞骨架显色正常 ,少数FAS染色阳性 ,末端脱氧核苷酸转移酶介导DUPT的缺口末端描记法 (TUNEL法 )全部阴性。 0区MAP2和NF2 0 0消失 ,1区MAP2和NF2 0 0显著异常 ,提示为不可逆的缺血损伤 ;2区MAP2和NF2 0 0有轻度改变 ,提示损伤可能为可逆性 ;3区细胞骨架正常而星形细胞增生明显 ,表明为损伤反应。结论暗神经元是脑缺血后的主要神经元变化形式之一 ,少数处于凋亡早期 ,多数则尚未凋亡。缺血细胞骨架损伤轻者 ,神经细胞功能有可能恢复 ;损伤重者 。
Objective By observing the cytoskeleton changes of seven infarcted human brains on autopsies,it was designed to find wheather the ischemic neurons and the dark neurons were reversible. Methods We selected 7 autopsy brains of the cerebral infarction patients. We devided each infarcted brain into four areas on the large section for each whole infarcted brain. We then examined the microtube associated protain 2 (MAP2) and 200 kd neurolfilaments(NF200) by immunohistochemistry. Results The area 0 with disappearance of MAP2 and NF200 represented a necrotic area. The area 1 with of NF200 and MAP2 dramatic abnormal changed of NF200, disclosed an irreversible area. The area 0 together with area 1 formed the central necrosis area. The area 2 with gently changes of MAP2 and NF200 and GFAP positive astrocyte hyperplasia, represented the possible penumbra in the reversible area. The area 3 with normal cytoskeleton and astrocyte hyperplasia disclosed the reflecting area and possible chronic penumbra. The dark neurons consisted of 80%～90% in the area 1,there were 40 %～50% in area 2, a few of them were positive with fuschin,alizerin and safracin staining ( FAS positive) and all of them were TUNEL negative. Conclusions The dark neurons might be considered one of the major neuronal changes following ischemia.A few of the irreversible dark neurons had FAS positive staining in the early apoptosis stage. Most of the dark neurons had no apoptosis. One part of the ischemic neuron with dramatic cytoskeleton changes was irreversible, whereas the other part of them with gentle changes of cytoskeleton was reversible.
出处
《中华神经科杂志》
CAS
CSCD
2000年第1期39-41,共3页
Chinese Journal of Neurology
基金
九五国家重点科技项目攻关计划!(969060221)
