摘要
目的建立测定福建组培金线莲中总黄酮含量的荧光光度法。方法根据黄酮类化合物与Al3+能形成稳定的荧光络合物,采用95%乙醇超声提取福建组培金线莲中的总黄酮,以槲皮素为对照品,用荧光光度法测定总黄酮含量。结果在选定的实验条件下,槲皮素浓度与荧光强度具有良好的线性关系,线性范围为0.002～0.400μg/mL,检出限为0.0004μg/mL,线性方程F=2002.6C+5.5296,相关系数r=0.9995,测得福建组培金线莲中总黄酮含量(按干燥品计算)为73.98μg/g,平均回收率为99.95%,相对标准偏差为0.92%(n=5)。结论本法操作简便、快速、准确,适用于福建组培金线莲中总黄酮的含量测定。
Objective To establish the Spectrofluorimetry of determining total flavanoid in tissUe cultured Anoectochilus roxburghii （ Wall. ） Lindl!from Fujian . Methods On the basis of reaction of total flavanoid with alumimum ion to form fluorescent material, using 95% ethanol by ultrasonic wave to extract total flavanoid in tissue cultured Anoectochilus roxburghii （ Wall. ） Lindl from Fujian, a fluorimetric method for determination of total flavanoid was proposed by taking quecetin as standard sample. Results Under the optimum conditions, a linear relationship was obtained between the fluorescence intensity and the concentration of quercetin in the range of 0.002 - 0.400 u g/mL, and the detection limit was 0.000 4 Ix g/mL, linearity relationship was F=2002.6C＋5.5296, correlativity was 0.9995, determination of total flavanoid in tissue cultured anoectochilus roxburghii （wall.）lindl from Fujian （according to the dry product calculation） was 73.98 u g/g, average rate of recovery was 99.95%, RSD was 0.92%（n=5）. Conclusion This method is simple, rapid, accurate which is suitable for determining total flavanoid in tissue cultured Anoectochilus roxburghii （wall.）lindl.from Fujian.
出处
《中国医药科学》
2012年第2期121-123,共3页
China Medicine And Pharmacy
基金
福建省教育厅资助项目(JB09287)
关键词
金线莲
福建组培
总黄酮
荧光光度法
Anoectochilus roxburghii （wall.）lindl
Tissue cultured from fujian
Total flavanoid
Spectrofluorimetry
