摘要
目的:探讨中药有效成分穿心莲内酯对白念珠菌生物膜分散细胞凋亡的影响。方法:Hoechst33258染色荧光显微镜检测白念珠菌生物膜细胞凋亡的形态;Rh123染色流式细胞仪检测白念珠菌生物膜细胞线粒体膜电位(MMP)变化;DHR染色流式细胞仪检测白念珠菌生物膜细胞内活性氧(ROS)水平。结果:1 000,100μmol.L-1的穿心莲内酯能诱导白念珠菌生物膜细胞核固缩、浓染致密,1 000,100,10μmol.L-1的穿心莲内酯能降低白念珠菌生物膜线粒体膜电位,提高细胞内ROS水平。结论:一定浓度的穿心莲内酯可诱导白念珠菌生物膜分散细胞凋亡。
Objective： To detect the effect of andrographolide on apoptosis of Candida albicans biofihn dispersion cells. Method ： The morphological changes of apoptotic C. albicans biofilm cells were observed by using Hoechst 33258 staining Fluorescence microscope; changes of mitochondrial membrane potential （MMP） of C. albicans biofilm cells were detected by rhodamine 123 staining flow cytometry; and reactive oxygen species （ROS） was detected by DHR staining flow cytometry. Result： 1 000, 100 μmol·L-1 of audrographolide could cause pyknosis and dense staining of C. albicans biofilm cells, 1 000, 100, 10μmol·L-1 of andrographolide could decrease MMP and increase ROS of C. alb＆ans biofilm cells. Conclusion ： Andrographolide of appropriate concentrations could induce apoptosis of dispersion cells of C. albicans biofilms.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第3期362-365,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81073127)
安徽省自然科学基金项目(090413092)
安徽省高校省级自然科学研究重点项目(KJ2010A220)
关键词
穿心莲内酯
白念珠菌
生物膜分散细胞
凋亡
andrographolide
Candida albicans
biofilm dispersion cell
apoptosis
