摘要
目的:检测重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠血清中中性粒细胞弹性蛋白酶(neutrophil elastase,NE)、白介素-6(interfilon-6,IL-6)的含量及胰腺病理学改变,探讨西维来司钠对大鼠SAP的治疗作用.方法:采用经十二指肠乳头逆行胆胰管注射5%牛磺胆酸钠的方法制备大鼠SAP模型;药物治疗组静脉输入西维来司钠,切取胰腺组织行病理学评分,测定血清中NE、IL-6及淀粉酶的含量.结果:牛黄胆酸钠胰胆管注射可以制作典型的急性胰腺炎模型,其血清淀粉酶升高、胰腺病理损伤符合急性胰腺炎表现.西维来司钠治疗组血清淀粉酶值及NE、IL-6含量较模型组降低,有显著性差异(3h:5636.22±713.57 vs 5835.75±681.52,16.99±3.28 vs 22.93±4.74,181.86±36.56 vs 281.82±30.79;6h:5743.44±624.93 vs 6253.66±533.99,23.63±4.47 vs 31.81±4.69,184.15±28.56 vs 319.39±21.73;12h:7098.93±698.42 vs 8420.74±779.72,24.46±5.02 vs 39.21±6.23,192.52±37.65 vs 354.21±23.72,均P<0.05);且药物治疗组胰腺损伤的组织病理学评分较模型组降低,有显著性差异(P<0.05).NE、IL-6与胰腺病理学评分相关性分析显示,血清中NE、IL-6含量与胰腺损伤程度呈正相关.结论:西维来司钠能够降低血清中NE、IL-6浓度,减轻胰腺组织的病理损伤,减轻SAP急性期的炎症反应.
AIM： To evaluate the therapeutic effect of sivelestat on severe acute pancreatitis （SAP） in a rat model by measuring the levels of serum neutrophil elastase （NE） and interleukin-6 （IL-6） and examining pancreatic pathological changes. METHODS： SAP was induced in rats by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Sivelestat was instilled continuously with an infusion pump in rats in the treatment group. Pancreatic pathological changes were evaluated by HE staining. The levels of serum NE and IL-6 were measured by ELISA. The level of serum amylase was measured using a biochemical analyzer. RESULTS： The level of serum amylase was higher and pancreatic pathological changes were obvious in SAP rats compared to control rats. The levels of serum amylase, NE and IL-6 at various time points were significantly lower in treated rats than in SAP rats （3 h： 5636.22 ± 713.57 vs 5835.75 ± 681.52, 16.99 ± 3.28 vs 22.93 ± 4.74, 181.86 ± 36.56 vs 281.82 ± 30.79; 6 h： 5743.44 ± 624.93 vs 6253.66 ± 533.99, 23.63 ± 4.47 vs 31.81 ± 4.69, 184.15 ± 28.56 vs 319.39 ± 21.73; 12 h： 7098.93 ± 698.42 vs 8420.74 ± 779.72, 24.46 ± 5.02 vs 39.21 ± 6.23, 192.52 ± 37.65 vs 354.21 ± 23.72, all P 0.05）. The score of pancreatic pathological changes was significantly lower in treated rats than in SAP rats （P 0.05）. Serum levels of NE and IL-6 had a positive correlation with the score of pancreatic pathology. CONCLUSION： Sivelestat could reduce serum levels of IL-6 and NE, mitigate pancreatic injury, and inhibit inflammatory reaction in rats with SAP.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第35期3579-3584,共6页
World Chinese Journal of Digestology
基金
河北省自然科学基金资助项目
No.C2008001284~~
