摘要
目的:探讨石菖蒲不同药效部位改善阿尔茨海默病(Alzheimer disease,AD)模型小鼠认知功能的机制。方法:雄性NIH小鼠,采用双侧海马CA1内注射淀粉样β蛋白(Aβ1-42,2 g/L,每侧2μL)制备AD小鼠动物模型,并以生理盐水模拟注射和不注射的正常组小鼠为对照。通过水迷宫测试,筛选出与两对照组均有显著差异的认知障碍小鼠,留作AD动物模型。AD动物模型又随机分为生理盐水灌胃组、石菖蒲水煎液灌胃组、石菖蒲去油水煎液灌胃组和石菖蒲挥发油灌胃组。每组6只,共4组。以生理盐水灌胃组为对照,石菖蒲不同药效部位灌胃组每天对应灌胃1次(0.2 g石菖蒲/10 g体重),连续3周。灌胃结束,Morris水迷宫检测小鼠的空间学习记忆能力,测定小鼠大脑和海马中一氧化氮合酶(nitric oxide synthase,NOS)活性,免疫组织化学方法检测脑中NOS的表达。结果:水迷宫测试结果表明,石菖蒲不同药效部位(水煎液和挥发油)灌胃2组模型小鼠与生理盐水灌胃组小鼠相比,60 s内跨越平台的次数和在目标象限的探索时间均明显增加(P<0.05);大脑和海马内NOS活性检测显示,石菖蒲不同药效部位灌胃3组AD模型小鼠和其对照组相比,大脑和海马内NOS活性下降,NOS阳性神经元数量明显减少(P<0.05)。结论:石菖蒲不同药效部位能显著改善Aβ1-42致AD模型小鼠的学习记忆能力,其疗效可能与降低大脑和海马中的NOS活性有关。
AIM： To investigate the effects of different fractions of Acori graminei rhizoma extracts on the learning and memory abilities in amyloid β-protein（Aβ）-induced Alzheimer disease（AD） mice.METHODS： Aβ1-42（2 g/L,2 μL for each lateral） was slowly injected into the CA1 area of bilateral hippocampus in male NIH mice.The mice in saline group and naive group served as controls.Morris water maze test was applied to evaluate the levels of cognitive deficits of the mice for determining the establishment of the AD model.The mice in AD group were further divided into AD plus saline,AD plus decoctum,AD plus decoctum without oil and AD plus essential oil groups（6 animals each）.Subsequently,all mice were gavaged with Acori graminei rhizome at a daily dose of 0.2 g/10 g BW for 3 weeks,except AD plus saline group.Three weeks later,behavior examination of the mice was performed by Morris water maze test.The activity of nitric oxide synthase（NOS） in the cerebrum and hippocampus were assayed.NOS in the brain were examined by immunohistochemistry.RESULTS： The results of Morris water maze test showed that the swimming time in target quadrant and the frequencies of acrossing platform in 60 s in AD plus Acori graminei rhizome groups were significantly longer than those in AD plus saline group（P0.05）.Compared with AD plus saline group,the activity of NOS in the cerebrum and hippocampus,and the quantities of the positive cells were significantly decreased in AD plus Acori graminei rhizome groups（P0.05）.CONCLUSION： The different fractions of Acori graminei rhizome extracts improve the learning and memory abilities in AD mice induced by Aβ1-42.This effect might be related to the decrease in NOS activity.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第1期159-162,167,共5页
Chinese Journal of Pathophysiology
基金
广东省科技计划项目(No.2010B060900085)
关键词
石菖蒲
阿尔茨海默病
淀粉样Β蛋白
一氧化氮合酶
Acori graminei rhizome
Alzheimer disease
Amyloid beta-protein
Nitric oxide synthase
