摘要
目的利用DTI测定犬肝纤维化模型的ADC值、FA值,评估肝纤维化程度,分析其与CT灌注参数及VEGF表达水平的相关性。方法健康中华田园犬15只,随机分成实验组(n=10)和对照组(n=5)。实验组犬经腹腔注射50%CCl4油溶液,辅以高脂饮食,建立犬肝纤维化模型。对2组犬每隔4周行MR DTI、CT灌注成像及肝穿活检。根据病理分期分组,分析各组MR DTI参数ADC值、FA值,并与CT灌注参数及VEGF表达水平进行对照。结果成功获得各期肝纤维化模型;随肝纤维化进展,ADC值逐渐降低(P<0.05),FA值总体呈上升趋势(P>0.05);ADC值与PVP、TLP呈正相关;VEGF表达水平随肝纤维化进展而明显增高(P<0.05)。结论对犬行腹腔注射CCl4油溶液辅以高脂饮食可成功模拟人类从肝细胞变性-肝纤维化-肝硬化的全过程;MR DTI能反映肝纤维化各期的血流动力学变化趋势;VEGF可能在慢性肝病所致肝纤维化过程中起重要作用。
Objective To measure the ADC and FA values of liver fibrosis of dog models with DTI,and to analyze the correlation with CT hemodynamic indexes and VEGF expression.Methods Fifteen healthy Chinese rural dogs were divided into experimental group（n=10） and control group（n=5）.Liver fibrosis was induced in dogs in experimental group by intraperitoneal injection of CCl 4 and fed with high-fat diet.MR DTI scan,CT perfusion scan and liver biopsies were performed every 4weeks.DTI parameters were measured according to the pathology,and the correlation with CT hemodynamic indexes and VEGF expression were analyzed.Results The dogs in experimental group were successfully induced into different degrees of liver fibrosis.ADC values declined gradually（P〈0.05）,while FA values increased（P〈0.05） with liver fibrosis deteriorated.ADC values were positively correlated with PVP and TLP values.The VEGF expression ascended significantly with liver fibrosis progressed（P〈0.05）.Conclusion The dog models that simulating human hepatic fibrosis can be induced successfully by CCl4and high fat diet.DTI can reflect the tendency of hemodynamic in different degrees of fibrosis.VEGF plays an important role in fibrogenesis procession.
出处
《中国医学影像技术》
CSCD
北大核心
2012年第2期216-220,共5页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金(30900359)
关键词
肝硬化
扩散磁共振成像
体层摄影术
X线计算机
灌注
狗
Liver cirrhosis
Diffusion magnetic resonance imaging
Tomography
X-ray computed
Perfusion
Dogs
