摘要
目的:筛选分离纯化红景天苷的最佳树脂及其工艺参数。方法:采用静态吸附-解吸附方法,以红景天苷的吸附率和解吸附率为评价指标,从21种不同型号的大孔树脂中筛选出最佳树脂;对树脂纯化工艺参数:上样液浓度、上样液体积、上样液流速、洗脱溶剂、洗脱溶剂用量进行优化。结果:LSA-30效果最好,吸附率和解吸率分别为63.86%和82.39%;其最佳工艺为:上样液浓度为每1 mL相当于原生药0.4 g,上样液体积(mL)与树脂重量(g)之比为4∶1,上样液流速为1.0～2.0 mL/min,10倍柱体积的30%乙醇洗脱。产品红景天苷的质量分数为10.7%,收率为70.6%。结论:该优选工艺可为红景天苷纯化的产业化生产提供依据。
Objective：To select the appropriate macroporous resin and optimize the process parameters for purifying salidroside in Rhodiola crenulata.Methods： The static adsorption and desorption capacities of salidroside on 21 macroporous resins were investigated.Several important parameters influencing purification efficiency including sample concentration,sample volume,adsorption rate,type and dosage of eluent were optimized.Results： LSA-30 showed good separation efficiency with adsorption and desorption rate of 63.8% and 82.39%,respectively.The optimum conditions for purifying process were as follows： the sample concentration was that 1 mL was equal to 0.4 g raw material.The ratio of sample volume（mL） to resin weight（g） was 4∶ 1.The adsorption rate was 1~2 mL/min.30% ethanol with 10 times of the bed volume was adopted as the eluent.Under the optimal conditions,the purity and yield rate of the obtained salidroside was 10.7% and 70.6%,respectively.Conclusion： The optimized process provides guidance for the industrial production of salidroside.
出处
《中药材》
CAS
CSCD
北大核心
2011年第12期1936-1939,共4页
Journal of Chinese Medicinal Materials
基金
中国科学院"科技支青工程"项目(2007)
关键词
红景天
红景天苷
大孔树脂
纯化
Rhodiola crenulata
Salidroside
Macroporous resin
Purification
