摘要
目的探讨自体皮肤成纤维细胞(Fibroblasts,Fbs)与毛发角蛋白(Hair keratin,HK)复合填充材料在面部除皱中的作用,为其在医学美容中的应用提供实验依据。方法采用消化分离法对人Fbs进行原代培养,并按不同比例与HK颗粒进行复合培养,台盼蓝排斥法检测细胞的存活率,MTT法检测细胞的增殖活力,ELISA法检测培养液中Ⅰ型胶原蛋白(CollagenⅠ,ColⅠ)的含量。将志愿者耳后部Fbs与HK颗粒共培养后回注至面部皱纹真皮层内,观察除皱效果。结果 HK与Fbs的比例为1∶2时,细胞的存活率、增殖活力及培养液中的ColⅠ含量均最高;志愿者术后4周,注射部位手感柔软,顺应性良好,无僵硬、挛缩等情况出现,皱纹的填充效果良好,外形改善明显。结论自体Fbs与HK复合填充材料能明显促进面部皱纹的修复,本研究为其临床应用提供了实验依据。
Objective To investigate the role of complex filling material consisting of autologous skin fibroblast （Fbs） and hair keratin （HK） in removal of facial wrinkles and provide an experimental basis for application of the material in medical cosmetology. Methods Fbs isolated from volunteers were subjected to primary culture by digestion method, then to complex culture with HK particles at various ratios, and determined for survival rate by trypan blue exclusion method, for proliferation activity by MTr method, and for collagen I （Col I ） content in medium by ELISA. The autologous Fbs extracted from opisthotic skins of 10 volunteers were co-cultured with HK particles and injected intradermally into the facial wrinkles to observe the wrinkle-removal effect. Results When the ratio of HK and Fbs was 1 ： 2, the survival rate and proliferation activity of Fbs as well as the Col I content in medium reached peak value. Four weeks after injection, the skins at injection sites of volunteers were soft and of good compliance, while no stiffness or contracture appeared, indicating good filling effect. The appearances of volunteers were improved significantly. Conclusion The complex filling material consisting of autologous skin Fbs and HK promoted the repair of facial wrinkles significantly, which provided an experimental basis for clinical application of the material.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第2期220-223,共4页
Chinese Journal of Biologicals
基金
吉林省科学技术厅重点项目资助课题(20080438)
