摘要
目的建立祛浊通痹方中落新妇苷含量的测定方法。方法采用快速液相色谱法(RPLC)。色谱柱为EclipsePlus C18柱(2.1 mm×100 mm,1.8μm),以甲醇-水-冰醋酸(30∶70∶0.2)为流动相,柱温为25℃,流速为0.2 mL/min,检测波长为290 nm。结果落新妇苷在0.105～0.525μg范围内与其峰面积呈良好线性关系(r2=0.999 3,n=5),平均回收率为101.29%,RSD=2.76%。结论本方法分析时间短且重复性和稳定性好,可用于祛浊通痹方的质量控制。
Objective To establish a method for determining astilbin in Quzhuo Tongbi Fang.Methods The method was carried out by RPLC with a Eclipse Plus C18 column（2.1 mm×100 mm,1.8 μm）,methanol-water-acetic acid（30∶70∶0.2） severed as the mobile phase.The column temperature was 25 ℃,the flow rate was 0.2 mL/min,and the detection wavelength was at 290 nm.Results Astilbin showed a good linear relationship at the range of 0.105-0.525 μg（r2=0.999 3,n=5）.The average recovery of astibin was 101.29%（RSD=2.76%）.Conclusion The method is reliable,stable and reproducible.It can be used for quality control of Quzhuo Tongbi Fang efficiently.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第2期47-48,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
"十一五"国家科技支撑计划(2007BAI20B06)
