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重组人粒细胞巨噬细胞集落刺激因子对烫伤大鼠创面愈合及新生血管化的影响 被引量:5

Effects of rhGM-CSF on scalding wound healing and neovascularization in rats
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摘要 目的:研究重组人粒细胞巨噬细胞集落刺激因子(recombinant human granulocyte-macrophage colony-stimulating factor,rhGM-CSF)对烫伤创面组织愈合及新生血管化的影响。方法:72只SD大鼠于背部制备直径3 cm的深Ⅱ度烫伤创面。根据创面不同处理方式将大鼠随机分为实验组(创面局部应用rhGM-CSF凝胶剂)和对照组(创面局部应用不含rhGM-CSF的凝胶基质),每组36只。分别于创面形成后当日和第1、3、7、10、14、21天观察创面并计算创面愈合率;免疫组织化学染色观察创面组织中新生血管内皮细胞表面标志物CD31表达,计算微血管密度。结果:自创面形成后第7天起,实验组创面愈合率明显高于对照组(P<0.01),实验组创面组织中CD31表达及微血管密度也均明显高于对照组(P<0.01)。结论:外用rhGM-CSF具有促进烫伤创面愈合作用,其机制可能与上调创面组织中CD31表达、使新生血管再生加速有关。 Objective:To evaluate the effect of rhGM-CSF on scalding wound healing and neovascularization.Methods:Deep Ⅱ degree burn wound was made in 72 SD rats on the back with diameter of 3 cm.Rats were randomly divided into experiment group(n=36,local wound was treated with rhGM-CSF gel) and control group(n=36,local wound were treated with gel matrix without rhGM-CSF).The process of wound healing was observed,and the percentages of wound closure were calculated on the day,the 1st,2nd,3rd,7th,10th,14th,and 21st day after scald.The expression of CD31,the surface maker of neovascular endothelial cells was detetcted within the wound sites by immunohistochemical staining,and the microvessel density was calculated.Results:The percentages of wound closure in experiment group were significantly higher than that in control group from the 7th day to the 21st day.Immunohistochemical detection revealed that the expression of CD31 and the microvessel density in experiment group were significantly higher than those in control group from 7th day to 21st day(P0.01).Conclusions:The treatment of rhGM-CSF may promote scalding wound healing in rats.The mechanism may be associated with upregulation of CD31 expression and acceleration of neovascularization.
出处 《蚌埠医学院学报》 CAS 2012年第1期17-19,共3页 Journal of Bengbu Medical College
关键词 伤口愈合 烫伤 重组人粒细胞巨噬细胞集落刺激因子 大鼠 wound healing scald recombinant human granulocyte-macrophage colony-stimulating factor rat
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