摘要
【目的】研究小花棘豆总生物碱的抑菌作用。【方法】通过体外抑菌试验研究小花棘豆总生物碱的抑菌活性、最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和影响小花棘豆总生物碱抑菌效果的因素。【结果】小花棘豆总生物碱对金黄色葡萄球菌、乳房链球菌、化脓链球菌和枯草芽孢杆菌有抑菌作用;其对乳房链球菌和化脓链球菌的MIC为6.3 mg/mL,对金黄色葡萄球菌的MIC为1.6 mg/mL,对枯草芽孢杆菌的MIC为0.8 mg/mL。生物碱在不同pH值下的抑菌活性发生变化;高温使其抑菌活性消失;紫外照射对其稳定性没有影响。【结论】小花棘豆总生物碱对部分革兰氏阳性菌有较好的抑菌作用。
[ Objective ] The purpose of this project aim to study the antimicrobial activities of total alkaloids for Oxytropis glabra DC. [ Method ] Through bacteriostasis experiments in vitro, antimicrobial activities of total alkaloids for Oxytropis glabra DC on bacteriostasis efficiency, minimal inhibitory concentration, minimal bactericidal concentration, and environmental effect were studied. [ Result ] The result showed that alkaloid of Oxytropis glabra DC had fairly bacteriostasis for Staphylococcus aureus, Streptococcus uberis, Streptococcus pyogene, Bacillus subtilis ; the MIC for Streptococcus uberis and Streptococcus pyogene was 6.3 mg/mL, the MIC for Staphylococcus aureus was 1.6 mg/mL, the MIC for Bacillus subtilis was 0.8 mg/ mL. The diversity of bacteriostasis efficiency of alkaloid happened in different power of hydrogen; the bacteriostasis material has bad heat stability; ultraviolet ray treatment does not affect its stability. [ Conclusion] Total alkaloids of Oxytropis glabra DC had obvious bacteriostasis effect for Gram - positive bacteria.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2012年第1期175-180,共6页
Xinjiang Agricultural Sciences
基金
新疆生产建设兵团博士基金专项(2008JC07)
关键词
小花棘豆
生物碱
抑菌
Oxytropis glabra DC
alkaloid
bacteriostasis
