摘要
目的 研究血清丙型肝炎病毒(HCV) 含量与HCV 致病的关系及HCV 含量与抗HCV和丙氨酸转氨酶(ALT) 的相关性。方法 以逆转录聚合酶链反应(RTPCR) 法对HCV 感染的受血及相关供血系列血清进行HCVRNA定量分析,同时检测ALT 与抗HCV。结果 致输血后HCV 感染的供血中,HCVRNA 平均含量为108 .6 拷贝/L;抗HCV 及ALT 的异常检出率随HCVRNA 滴度升高而增加。结论 低含量HCV 供血即可导致输血后感染,病毒含量与抗HCV( +) 及ALT 水平有显著正相关,采用ALT 加抗HCV 联合筛检供血可明显降低输血后HCV
Objective To study the relationship between the hepatitis C virus (HCV) RNA level the level of alanine aminotransferase (ALT) as well as anti HCV in the sera of HCV infected blood recepients and their related blood donors. Methods The serum HCV RNA was detected by neverse transcription polymetase chain reaction (RT PCR) quantitatively and the levels of ALT and anti HCV were simultaneously detected. Results In the sera of donors which caused posttransfusion HCV infection, the mean level of HCV RNA were 10 8.6 copies/L. The positive rate of anti HCV in serum was increased in the patients with higher HCV RNA level. There was a strong correlaton between the HCV RNA level and ALT value. Conclusion The results suggested that posttransfusion HCV infection could be caused by transfusing blood with low HCV RNA level and exclusion of donated blood for elevated ALT or positive anti HCV could reduce the incidence of posttransfusion hepatitis C infection.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2000年第1期37-39,共3页
Chinese Journal of Infectious Diseases
基金
全军95 医药攻关课题基金!资助(96L049)
关键词
丙型肝炎病毒
HCV
输血后感染
血清病毒
Hepatitis C virus
Blood donor
Quantity of hepatitis C virus RNA
Antibody to hepatitis C virus
