摘要
目的通过比较食管癌组织和癌旁组织中存活蛋白的表达情况,探讨凋亡抑制因子存活蛋白与食管癌之间的关系。方法 T-A克隆制备存活蛋白定量PCR标准品pTA/存活蛋白;采用荧光定量聚合酶链反应(FQ-PCR)和免疫组织化学法检测33例经病理证实的食管癌组织(实验组)及其相应的癌旁组织(对照组)中存活蛋白的mRNA和蛋白质表达水平,并分析存活蛋白表达与食管癌临床病理特征间的关系。结果 FQ-PCR结果显示,实验组中存活蛋白的阳性表达率为81.82%(27/33),对照组中阳性表达率为39.40(14/33),两组比较差异有统计学意义(P<0.05);免疫组织化学结果在实验组和对照组中的阳性率与FQ-PCR结果一致,但对照组中的阳性细胞多为炎症细胞(非上皮细胞);存活蛋白的表达与患者年龄、性别及肿瘤分化程度无关(P>0.05),与肿瘤分期和淋巴结转移高度相关(P<0.05)。结论存活蛋白在食管癌组织中的阳性表达可能与肿瘤发生、浸润和淋巴结转移密切相关。
Objective To observe the correlation between survivin and the esophageal cancer by comparing the expression level of survivin in the cancer tissue and the adjacent tissue.Methods Recombinant plasmids pTA/survivin used as the standard plasmids in quantitative real-time PCR was constructed.Quantitative real-time PCR and immunohistochemistry were used to evaluate the endogenous expression level of survivin mRNA and protein in 33 cases of esophageal cancer tissues（experimental group） confirmed by pathology and their corresponding adjacent tissues（control group）.And the relationship between the expression level of survivin and the clinicopathologic parameters of esophageal cancer was also analyzed.Results FQ-PCR results showed that the positive expresstion rate of survivin in the experimental group was 81.82%（27/33）,the control group was 39.4%（14/33）,there was significant difference between the two groups when analyzed by the statistical software（P0.05）.The immunohistochemical results in the two groups were consistent with the FQ-PCR,but the positive cells of the control group were mostly inflammatory cells not the epithelial cells.There was no significant correlation between survivin expression and age,sex and tumor differentiation grade（P0.05）,but had significant relationship with tumor stage and lymph node metastases（P0.05）.Conclusion The expression of survivin in esophageal cancer tissue may be associated with tumorigenesis,invasion and lymph node metastasis.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第5期432-434,F0002,共4页
Chongqing medicine
基金
四川省卫生厅科研基金资助项目(030016)
关键词
食管肿瘤
存活蛋白
聚合酶链反应
esophageal neoplasms
survivin
polymerase chain reaction
