摘要
目的:建立五灵脂药材的HPLC指纹图谱。方法:采用HPLC法,色谱柱Diamonsil ODS C18(4.6 mm×250 mm,5μm);流动相乙腈(A)-0.5%冰醋酸水溶液(B);梯度洗脱(0～15 min,85%～65%B,15～35 min,65%～40%B,35～40min,40%～25%B,40～60 min,25%～10%B,60～65 min,10%～0%B;检测波长265 nm;流速0.8 mL.min-1;柱温室温。结果:建立了五灵脂药材的HPLC指纹图谱,标定了14个共有峰,并用对照品指认了其中3个峰,10批样品的相似度均大于0.90。方法的精密度、稳定性和重复性良好。结论:建立的五灵脂药材指纹图谱特征性及专属性强,可为其质量控制和药效物质基础研究提供参考。
Objective：To establish the HPLC fingerprint of Faeces Trogopterori（FT）.Method： The separation was performed on a Diamonsil ODS C18 column（4.6 mm×250 mm,5 μm） and the mobile phase consisted of acetonitrile（A）-0.5% aqueous acetic acid（B）,with an optimized gradient program of 0~15 min,85%-65% B,15-35 min,65%-40% B,35-40 min,40%-25% B,40-60 min,25%-10% B,60-65 min,10%-0% B.The flow rate was 0.8 mL·min-1and the wavelength was set at 265 nm.The experiment was performed under room temperature.Result： The HPLC fingerprint of FT was established,in which 14 co-peaks were searched out and 3 peaks were identified with reference substances.All of the similarities were greater than 0.90.The precision,stability and repetition were good.Conclusion： The characteristic and specialization of the fingerprint were good,which can provide the basis for its quality control and effective substance.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期98-100,共3页
Chinese Journal of Experimental Traditional Medical Formulae
