摘要
目的:探讨变应性鼻炎患者外周血Treg/Th17细胞相关转录因子及血清细胞因子表达的变化。方法:采用视觉模拟量表(VAS)评估变应性鼻炎患者(18例,实验组)症状的严重程度。用RT-PCR法检测实验组与对照组(12例健康体检者)外周血Treg/Th17细胞相关转录因子FOXP3、RORC的表达水平,ELISA法检测2组细胞因子TGF-β与IL-17的表达水平。结果:①中重度变应性鼻炎患者VAS评分为8.5±0.8,全体变应性鼻炎为6.2±1.6;②实验组FOXP3mRNA、TGF-β表达量均明显低于对照组(均P<0.01);RORC mRNA、IL-17表达量均高于对照组(均P<0.01)。结论:变应性鼻炎患者Treg/Th17细胞失衡可能在其发病中起作用。
Objective：To investigate the changes of Treg/Th17 cells related transcription factors（FOXP3,RORC） and cytokines（TGF-β,IL-17） in the peripheral blood in patients with allergic rhinitis.Method：Use visual analogue scale（VAS） to assess the allergic rhinitis severity of patients.Eighteen patients with allergic rhinitis and 12 healthy control subjects were consecutively enrolled.Peripheral blood samples were obtained from all patients and healthy control subjects（after prick test）.The mRNA expressions of key transcription factors（RORC,FOXP3） in PBMCs were measured by RT-PCR,and the concentrations of TGF-β,IL-17 in plasma were measured by ELISA.Result：Patients with moderate/severe persistent rhinitis have a VAS of 8.5±0.8,and total patients have a VAS of 6.2±1.6.Compared with healthy control subjects,the mRNA expressions of FOXP3 and the concentrations of TGF-β in plasma were significantly lower in patients with allergic rhinitis（P0.01） while the mRNA expressions of RORC and the concentrations of IL-17 in plasma were significantly higher in allergic rhinitis group（P0.01）.Conclusion：The imbalance of Treg/Th17 cells existed in allergic rhinitis patients,which may contribute to the proceeding of allergic rhinitis.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第5期209-211,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
