摘要
采用ICP MS、凝胶过滤层析及超滤技术 ,通过动物实验研究了长时间、低剂量镧作用后 ,镧在Wistar大鼠肝脏中的代谢累积及其物种分布。结果表明 ,随着给药剂量的增加及作用时间的增长 ,镧在肝脏中的累积量有规律地增大 ;停止给药一段时间后 ,积累在肝脏中的镧有不同程度的代谢 ,其代谢速度很慢 ;累积在肝脏中的镧主要分布于分子量大于 6 0 0 0 0的水溶性蛋白中 ;经SephacrylS 2 0 0层析分离得到的六个蛋白洗脱峰中均含有稀土 ,在第一个洗脱峰中含量最大 ,约占肝脏含镧大分子蛋白 (分子量大于 6 0 0 0 0 )总量的 88%
The metabolic accumulation and species of lanthanum in Wistar rat liver were investigated by ICP MS, gel exclusion chromatography and ultrafiltration after the rats were fed by low dose of lanthanum for a long time. It was found that the content of La in the liver increased regularly with arise of dose and time of drug delivery. After the administration was stopped for a certain time a part of lanthanum in the liver was metabolized, but the metabolic rate was very slow. The lanthanum in rat liver was distributed in the soluble protein with molecular weight of more than 60000 mostly. Rare Earth existed in the six elution peaks separated by Sephacryl S 200. The amount of lanthanum in the first elution fraction is the largest, which was 88 percent in the whole content of lanthanum in proteins with molecular weight more than 60000.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2000年第2期273-278,共6页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金! (No .2 9890 2 80 4)
国家博士后科学基金
