摘要
目的探讨牛初乳短链胰岛素样生长因子(Bct-IGF)对脂蛋白(a)[Lp(a)]诱导的肾小球系膜细胞(GMCs)增生与转化生长因子-β1(TGF-β1)表达的影响。方法将GMCs分为6组培养:对照组,Lp(a)组和不同浓度的Bct-IGF组,后5组培养时均加入5.0 mg.L-1Lp(a),后4组培养时分别加入100μg.L-1、200μg.L-1、400μg.L-1、800μg.L-1Bct-IGF。利用四甲基偶氮唑蓝(MTT)法测定细胞增殖率,末端转移酶介导的dUTP缺口末端标记法(TUNEL)测定GMCs凋亡率;ELISA法测定培养上清TGF-β1水平,反转录-PCR法检测TGF-β1 mRNA表达。结果与400μg.L-1Bct-IGF组比较,Lp(a)组、100μg.L-1Bct-IGF组、200μg.L-1Bct-IGF组GMCs细胞增殖率均明显增高,差异有统计学意义(Pa<0.05);800μg.L-1Bct-IGF组较之降低,但差异无统计学意义(P>0.05)。与400μg.L-1Bct-IGF组比较,LP(a)组、100μg.L-1Bct-IGF组、200μg.L-1Bct-IGF组GMCs细胞凋亡率均明显降低,差异有统计学意义(Pa<0.05);800μg.L-1Bct-IGF组较之增高,但差异无统计学意义(P>0.05)。Lp(a)组培养上清中TGF-β1水平显著高于400μg.L-1Bct-IGF组(P<0.01);Lp(a)组培养细胞TGF-β1 mRNA表达显著高于400μg.L-1Bct-IGF组(P<0.01)。结论 Lp(a)可以促进GMCs增生及TGF-β1的过表达,Bct-IGF可以抑制Lp(a)诱导的GMCs的异常增生,促进Lp(a)诱导的GMCs的凋亡。
Objective To observe the effects of bovine colostrums insulin-like growth factor（Bct-IGF） on proliferation of glomerular mesangial cells（GMCs） and expression of transformation growth factor β1（TGF-β1）after cultured with lipoprotein（a）. Methods GMCs were divided into 6 groups to culture：control group,Lp（a） group,and different concentration Bct-IGF groups,in which 5.0 mg·L-1 Lp（a） were added to the last 5 groups,and 100 μg·L-1,200 μg·L-1,400 μg·L-1,800 μg·L-1 of Bct-IGF were added to the last 4 groups,respectively.Then the rate of effective apoptosis was detected with transferase mediated d-UTP nick end labeling method,the rate of proliferation was detected with 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide dye method,the rate of TGF-β1 was detected with enzyme-linked immunosorbent assay,and the expression of TGF-β1 was detected with reverse transcription polymerase chain reaction. Results At the end of the experiment,the rates of proliferation in Lp（a） group,100 μg·L-1 Bct-IGF group,200 μg·L-1 Bct-IGF group were significantly higher than that in the 400 μg·L-1 Bct-IGF group（Pa0.05）;800 μg·L-1 Bct-IGF group was lower than that in the 400 μg·L-1 Bct-IGF group,but the difference was no significant（P0.05）.The rates of apoptosis in Lp（a） group,100 μg·L-1 Bct-IGF group,200 μg·L-1 Bct-IGF group were significantly lower than that in the 400 μg·L-1 Bct-IGF group（Pa0.05）;800 μg·L-1 Bct-IGF group was higher than that in the 400 μg·L-1 Bct-IGF group,but the difference was no significant（P0.05）.The le-vel of TGF-β1 and the expression of TGF-β1 in Lp（a） group were significantly higher than those in the 400 μg·L-1 Bct-IGF group（P0.01）. Conclusions Lp（a） can enhance the proliferation of GMCs,and the expression of TGF-β1.Bct-IGF can inhibit the over proliferation and enhance the apoptosis of GMCs induced by Lp（a）.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2012年第5期340-342,377,共4页
Journal of Applied Clinical Pediatrics
基金
深圳市科技计划项目(200802062)
