摘要
应用PCR方法,从郑州、福建、浙江金华和宁波猪肺脏中分别扩增出4段PCMV gB基因,并将其分别克隆入pMD18-T载体,经蓝白斑筛选和PCR鉴定,将阳性克隆进行序列分析并构建系统进化树。序列分析表明,其gB基因全长为2 580bp,编码860个氨基酸,与PCMV其他序列相比,其同源性在96.1%～99.7%,与β疱疹病毒亚科中其他常见毒株同源性在25.9%～38.1%;推导的氨基酸序列,有11个半胱氨酸,17个潜在N-糖基化位点,其裂解位点是RYKR;系统进化树分析发现,推导的氨基酸序列出现两个分支,而且来自上述4个地区PCMV gB糖蛋白氨基酸序列处在不同的分支中。
Porcine cytomegalovirus（PCMV） gB gene was amplificated from the pig lung of Zhengzhou,Fujian,Jinhua and Ningbo of Zhejiang province with PCR,and was cloned into pMD18-T vector,after blue-white selection and identification of PCR,the positive clone sequence was analysed and phylogenetic tree was constructed.The results showed that the gB gene was 2 580 bp in length,encoding 860 amino acids,and in comparison with other gB gene sequence with PCMV,the homology of the nucleotide sequence was 96.1%~99.7%,with other common strains in Beta herpersvirus,the homology of the nucleotide sequence was 25.9%~38.1%.The deduced amino acids sequence contains 11 cysteine residues,17 potential N-linked glycosylation motifs,as well as potential cleavage site RYKR was identified.Phylogenetic tree analysis revealed that the deduced amino acids sequence branched into two,and the four sequences crossed different branches.
出处
《中国兽医杂志》
CAS
北大核心
2012年第2期17-21,共5页
Chinese Journal of Veterinary Medicine
