摘要
目的:对四逆汤传统法、药典收录的水提醇沉法、各单味药分提合并法制备的四逆汤中甘草苷、甘草酸含量进行比较。方法:采用HPLC测定3种不同的方法提取液中甘草苷、甘草酸的含量,C18色谱柱,流动相乙腈-0.05%磷酸水,梯度洗脱,柱温25℃,检测波长237 nm,流速1.0 mL.min-1,进样量10μL。结果:甘草苷在传统汤剂中含量为2.57 mg.g-1,经方合剂中的含量为2.21 mg.g-1,单味配方合并液中含量为3.58 mg.g-1;甘草酸在传统汤剂中含量为1.96 mg.g-1,经方合剂中的含量为1.84 mg.g-1,单味配方合并液中含量为3.04 mg.g-1。结论:四逆汤单提合并法中甘草苷、甘草酸含量高,且各制法的四逆汤中甘草苷、甘草酸含量均有显著性差异,为四逆汤提取方法研究提供依据。
Objective： To compare the contents of licorice glucoside and glycyrrhizic acid in Sini Decoction processed by traditional extraction method, water extraction and alcohol precipitation method included in Pharmacopoeia and the single herb separately extracted then combined method. Method： To determine the the contents of licorice glucoside and glycyrrhizic acid was determined by three extraction method. Separation was carried out on C18 column. A linear solvent gradient elution is used and solvent A was acetonitrile, and solvent B is water containing 0.05% phosphoric acid. The flow rate was 1.0 mL.min -1 at 25℃ and the detector was set at 237 nm. Result： The content of licorice glucoside was 2.57 mg. g-1 in the traditional decoction, 2.21 mg. g-1 in the mixture and 3.58 mg. g-1 in the single herb formula combined solution in a prescription. The content of glycyrrhizic acid was 1.96 mg . g-1 in the traditional decoction, 1.84 mg .g-1 in the mixture and 3.04 mg .g- 1 in the single herb formula combined solution in a prescription. Conclusion： The contents of licorice glucoside and glycyrrhizic acid in the single herb formula combined solution in a prescription is higher than the others. The contents of licorice glucoside and glycyrrhizic acid is significantly different in the preparation processed by different extraction methods which can provide references for Sini decoction extraction technology.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期71-74,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省高等学校科技计划项目(Z09LF31)
关键词
四逆汤
甘草苷
甘草酸
高效液相色谱
Sini decoction
licorice glucoside
glycyrrhizic acid
HPLC
