摘要
目的:探讨脑钠肽(BNP)及钠肽受体(NPR-A)在慢性非细菌性前列腺炎(CNP)大鼠脊髓背角神经节(DRG)中的表达情况,以及与CNP引起的慢性病理性疼痛的关系。方法:健康清洁级SD大鼠40只,实验分CNP模型组30只及假手术组10只,采用完全弗氏佐剂法对SD大鼠进行CNP模型的建立,前列腺组织HE染色。采用荧光定量PCR法检测BNP、NPR-A、GAPDH在各实验组大鼠L5-S2 DRGs中的表达情况。结果:HE染色发现随着造模时间的延长,炎症程度越剧烈。BNP在造模3、7、10 d组的表达量分别为假手术组的(2.16±0.35)、(1.61±0.21)、(1.32±0.36)倍。NPR-A在造模3、7、10 d组的表达量分别为假手术组的(2.75±0.06)、(2.15±0.15)、(1.04±0.13)倍,其中3 d和7 d与假手术组比较有统计学意义(P<0.05)。10 d组与假手术组无统计学差异。结论:BNP及NPR-A在SD大鼠L5-S2 DRGs中表达,并且在炎症时表达上调。BNP及NPR-A可能参与了CNP引起疼痛的机制。
Objective： To investigate the expressions of brain natriuretic peptide （BNP） and natriuretic peptide receptor （NPR-A） in the cord dorsal horn ganglion （DRG） of rat models of chronic nonbacterial prostatitis （CNP） and the relation of BNP and NPR-A with CNP-induced chronic pain. Methods： We established CNP models in 30 healthy clean SD rats using Freund＇s complete adjuvant, and included another 10 in a sham-operation group. The prostate tissues were subjected to HE staining, and the expressions of BNP and NPR-A in the L5-S2 DRGs were detected by real-time PCR. Results ： Higher degree of inflammation was related to longer modeling time. At 3, 7 and 10 days, the expressions of BNP in the CNP models were 2.16 ± 0. 35, 1.61 ± 0.21 and 1.32 ± 0.36, and those of NPR-A were 2.75 ± 0. 06, 2.15 ± 0.15 and 1.04 ± 0. 13, respectively, significantly higher at 3 and 7 days as compared with the sham-operation group （P 〈 0.05 ） , but with no statistically significant difference at 10 days. Conclusion： BNP and NPR-A are expressed in the L5-S2 DRGs of SD rats and their expressions can be upregulated by CNP. BNP and NPR-A may be involved in the mechanisms of CNP-induced pain. Natl J Androl, 2012, 18 （3） ： 204 -207
出处
《中华男科学杂志》
CAS
CSCD
2012年第3期204-207,共4页
National Journal of Andrology
基金
江西省卫生厅科技计划(20121030)~~
