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新生籽鹅组织qRT-PCR分析中内参基因的选择 被引量：5

Validation of reference genes for gene expression analysis in neonatal Zi goose tissues using quantitative real-time PCR
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摘要 分别以1日龄健康籽鹅肝脏、肾脏、心脏、肌肉和卵巢为研究对象,应用实时定量RT-PCR技术,探讨28SrRNA、18SrRNA、GAPDH、ACT、HPRT1、SDH和TUB等7个内参基因mRNA的表达水平。经过geNorm和NormFinder程序分析,结果显示7个内参基因稳定度由高到低依次为GAPDH=HRPT1>28SrRNA>TUB>SDH>ACT>18SrRNA;基因表达的稳定值分别为0.215(GAPDH),0.215(HRPT1),0.339(28SrRNA),0.471(TUB),0.721(SDH),0.888(ACT),1.177(18SrRNA);表明GAPDH和HRPT1这2个内参基因适合用于目的基因表达的校正。 The purpose of the present study was to investigate the expression of reference genes 28S rRNA,18S rRNA,GAPDH,ACT,HPRT1,SDH and TUB in 1-day-old Zi goose liver,kidney,heart,muscle and ovary by qRT-PCR.The results showed that stability of the seven reference genes from high to low was GAPDH=HRPT128S rRNATUBSDHACT18S rRNA,and the value were 0.215（GAPDH）,0.215（HRPT1）,0.339（28S rRNA）,0.471（TUB）,0.721（SDH）,0.888（ACT）,1.177（18S rRNA） respectively by geNorm and NormFinder analysis.It is suggested that GAPDH and HRPT1 are suitable reference genes and could be used to normalize mRNA levels between different samples.
作者 王忠伟 郭景茹 王建发 臧琳 郭爽 杨焕民
机构地区 黑龙江八一农垦大学动物科技学院 吉林大学畜牧兽医学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2012年第3期427-431,共5页 Chinese Journal of Veterinary Science
基金 黑龙江省科技厅资助项目(GB06B204-4) 黑龙江农垦总局资助项目(HNKXIV-08-05a)
关键词 内参基因 实时定量RT-PCR geNorm程序 NormFinder程序 籽鹅 reference gene qRT-PCR geNorm NormFinder Zi gooes
分类号 Q78 [生物学—分子生物学] S858.33 [农业科学—临床兽医学]
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参考文献11

  • 1陈凤花,王琳,胡丽华.实时荧光定量RT-PCR内参基因的选择[J].临床检验杂志,2005,23(5):393-395. 被引量：59
  • 2王兰芳,乐国伟,施用晖,马西艺.日粮核苷酸对早期断奶小鼠生长发育的影响[J].无锡轻工大学学报（食品与生物技术）,2003,22(4):18-22. 被引量：21
  • 3Vandesompele J,De Preter K,Pattyn F,et al.Accuratenormalization of real-time quantitative RT-PCR databy geometric averaging of multipleinternal controlgenes[J].Genome Biol,2002,3(7):1-11.
  • 4Claus L A,Jens L J,Torben F O.Normalization of re-al-time quantitative reverse transcription-PCR data:Amodel-based variance estimation approach to identifygenes suited for normalization,applied to bladder andcolon data sets[J].Cancer Res,2004,64:5245-5250.
  • 5Infante C,Matsuoka M P,Asensio E,et al.Selection ofhouse-keeping genes for gene expression studies in lar-vae from flatfish using real-time PCR[J].BMC MolBiol,2008(9):28.
  • 6韦宜峰,黄光武.实时荧光定量PCR在鼻咽癌中的应用[J].中国医学文摘（肿瘤学）,2003,17(2):168-170. 被引量：2
  • 7Suzuki T,Higgins P J,Crawford D R.Control selectionfor RNA quantitation[J].Biotechniques,2000,29(2):332-337.
  • 8AnnB N,Claus B J,Susanna C,et al.Selection of refer-ence genes for gene expression studies in pig tissues u-sing SYBR green PCR[J].BMC Mol Biol,2007(8):67.
  • 9Radonic A,Thulke S,Mackay I M,et al.Guideline toreference gene selection for quantitative real-timePCR[J].Biochem Biophy Res Commun,2004,313(4):856-862.
  • 10Solomon M,Arpad B G,Szilard B,et al.Quantitativeevaluation and selection of reference genes in mouseoocytes and embryos cultured in vivo and in vitro[J].BMC Dev Biol,2007(7):14.

二级参考文献58

  • 1Gil A, Sanchez-Medina F. Acid-soluble nucleotides of cow's, goat's and sheep's milks, at different stages of lactation[J]. J Dairy Res, 1981, 48:35-44.
  • 2Gil A, Sanchez-Medina F. Acid-soluble nucleotides of human milk at different stages of lactation[J]. J Dairy Res, 1982, 49:301 - 307.
  • 3Uauy R. Effect of dietary nucleotides on growth and maturation of the developing gut in the rat[J]. Journal of Pediatric Gastroenterology and Nutrition, 1990, 10(4) : 497 - 503.
  • 4Torres M I, Fernandez M I, Gil A, et al. Dietary nucleotides have cytoprotective properties in rat liver damaged by thioacetamide[J]. Life Science, 1998, 62(1) : 13 - 22.
  • 5Nunez MC, Ayudarte M W, Gil A, et al. Effect of dietary nucleotides on the fatty acid composition of rat liver microsomes[J]. Archives Internationals de physiologic, de biochemie et de biophysique, 1993, 101(2) : 123 - 128.
  • 6Jyonouchi H, Zhnag-Shanbhag L, Yokoyama H. Nucleotide-free diet impairs T-helper cell functions in antibody production in response to T-dependent antigens in normal C57B1/6 mice[J]. J Nutr, 1994, 124:475 - 484.
  • 7Lecce J G, Armstrong P C, Crawford P C, et al. Nutrition and management of early weaned piglets: Liquid versus dry feeding[J]. J Anim Sci, 1979, 48: 1007.
  • 8Sonoda T, Tatibana M. Metabolic rate of pyrimidines and purines in dietary nuclic acids ingested by mice[J]. Biochem Biophys Acta, 1978, 521: 55-56.
  • 9Lopez-Navarro A T, Gil A, Sanchez Poso A. Deprivation of dietary nucleotides results in a transient decrease in acid-soluble nucleotides and RNA concentration in rat liver[J]. J Nutr, 1995, 125: 2090- 2095.
  • 10Lopez-Navarro A T, Bueno I D, Gil A, et al. Morphological changs in hepatocytes of rats deprived of dietary nucleotides[J].Bri J Nutr, 1996, 76(4) :579 - 589.

共引文献146

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二级引证文献49

中国兽医学报
2012年 第3期

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