摘要
为分析牦牛源多杀性巴氏杆菌外膜蛋白H的免疫保护效果,将纯化的牦牛源多杀性巴氏杆菌重组外膜蛋白H(rOmpH)制备成疫苗,用该重组疫苗和全菌灭活疫苗皮下免疫小鼠。首免后每周采血1次,用间接ELISA方法检测其抗体动态变化,并于三免后第9天用2LD50的分离株进行攻毒,比较两者的保护力。结果显示,全菌灭活疫苗组小鼠30h后死亡率为20%,重组疫苗组小鼠30h后100%死亡,PBS对照组小鼠24h后100%死亡。结果表明,全菌灭活疫苗具有保护力,而重组蛋白疫苗保护性微弱。
To evaluate the immunogenicity of outer member protein H（OmpH） of yak Pasteurella multocida,vaccines were prepared with the purified recombinant OmpH（rOmpH） of the P.multocida.Mice were hypodermically immunized with the rOmpH vaccine and inactivated whole bacteria vaccine,with PBS as control.Sera were collected weekly from the mice and antibody responses were detected by indirect ELISA.The immunized mice were challenged with 2 LD50 P.multocida on day 9 after the third immunization to observe the protective efficacy.The results showed that 20% of the inactivated whole bacteria vaccine groups died after 30 h post-challenge,and 100% of the recombinant vaccine groups died after 30 h,and 100% of the PBS groups died after 24 h.The results showed that inactivated whole bacteria vaccine was shown to induce higher immune protection,but the rOmpH vaccine has lower immune protection.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2012年第3期290-293,共4页
Chinese Veterinary Science
基金
新疆农垦科学院科技引导计划项目(YYD201107)
新疆农垦科学院青年基金项目(YQJ2009-02
YQJ201110)
