摘要
目的探讨发热伴粒细胞血小板减少综合征布尼亚病毒(Severe fever with thrombocytopenia syndrome bunya-virus,SFTSV)在细胞分离培养时表现的特异性。方法收集发病一周以内发热伴粒细胞血小板减少综合征患者的血液,首先做血清学检测排除汉坦病毒(Hantavirus,HV),然后将样本采用多种细胞培养的方法进行病毒分离。最后采用逆转录(RT-PCR)方法对病毒进行鉴定。结果从我省发热伴出血征候群血液样本分离出病毒12株,经鉴定均为新布尼亚病毒。结论我省东部山区及丘陵地带有新布尼亚病毒流行,其临床症状及体征与肾综合征出血热极为相似。但体外细胞培养及核苷酸序列却存在着明显的差别。
To explore the specificity of Severe fever with granulocyte thrombocytopenia syndrome virus in many cells.Blood samples of patients with fever and leukothrombopenia for no more than one week was collected and had a serologic test for Hantavirus,then isolated the virus by using different kinds of cells.RT-PCR was adopted to identify virus in our research.Twelve virus strains were separated from samples of fever and bleeding blood symptoms in Liaoning province.The virus was identified SFTSV.It has had an epidemic of bunyaviridae virus in mountain district and hills in east of Liaoning Province.It is similar with hemorrhagic fever with renal syndrome at clinical symptoms and signs,but there is obvious distinction between them when culturing in cells.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第3期237-240,共4页
Chinese Journal of Zoonoses
基金
艾滋病和病毒性肝炎等重大传染病防治项目辽宁及周边省(市)传染病病原谱流行规律研究(2009ZX10004-209)
辽宁省科技厅卫生安全重点实验室项目(2007403016)联合资助
