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姜黄素对人绒毛膜癌JAR细胞增殖和细胞周期的影响 被引量：2

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摘要 目的研究姜黄素对人绒毛膜癌JAR细胞增殖抑制及细胞周期的影响,并探讨cyclin A及CDK2蛋白表达水平的变化。方法用不同浓度的药物作用于JAR细胞,采用MTT检测药物对细胞的增殖抑制能力的影响,采用流式细胞仪检测药物对细胞周期的影响,Western-blot检测cyclin A、CDK2的蛋白表达水平变化。结果姜黄素能明显抑制JAR细胞增殖,且具有明显的剂量和时间依赖性,流式细胞术检测显示一定浓度药物作用细胞,可将细胞周期阻滞于S期,Westernblot检测发现药物能下调cyclin A及CDK2蛋白的表达。结论姜黄素能显著抑制JAR细胞增殖,使细胞周期阻滞于S期,其机制可能是通过下调cyclin A及CDK2蛋白表达水平所致。
作者 庞江琳 陈杰 李英勇
机构地区 广东医学院附属医院 广东医学院
出处 《时珍国医国药》 CAS CSCD 北大核心 2012年第3期679-680,共2页 Lishizhen Medicine and Materia Medica Research
基金 广东省中医药局建设中医药强省科研课题(No.1060045)
关键词 姜黄素 JAR细胞 细胞周期 CYCLINA CDK2
分类号 R285.6 [医药卫生—中药学]
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时珍国医国药
2012年 第3期

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