摘要
目的研究树突状细胞（DC）疫苗对培养的慢性乙型肝炎（CHB）患者细胞因子诱导的杀伤细胞（CIK）免疫功能的调节作用。方法采用体外细胞培养的方法培养30例CHB患者CIK细胞，分为DC疫苗组和无DC疫苗组，培养14d后用流式细胞术检测各组ClK中CD3’CIM’、CD3’CD8’及CD3’CD56’T细胞的所占比例，ELISA方法检测培养上清中自细胞介素-12（IL-12）、γ干扰素（IFN-1）及白细胞介素-4（IL4）的浓度。结果DC疫苗组CD3^＋CIM^＋、CD3^＋CD8^＋及CD3^＋CD56^＋T细胞所占比例分别为18．27％、64．36％和20．00％，无DC疫苗组则分别为17．79％（P〉0．05）、54．69％（t=4．130，P〈0．01）和13．39％（t=5．601，P〈0．01）。DC疫苗组的CIK培养上清中IL．12、IL4及IFN-1的浓度分别为（177．82±130．06）、（31．774-9．52）、（86．99±56．30）ng/L，无DC疫苗组分别为（80．83±50．15）n∥L（t=3．811，P〈0．01）、（40．33±19．74）ng／L（t=2．141，P〈0．05）和（42．07±19．68）ng／L（t=4．125，P〈0．01）。结论CIK细胞培养中加入DC疫苗进行诱导，增强了所培养CIK细胞的杀伤活性。
Objective To investigate the regulation of the immune function of cytokine-induced killer cells（CIK） by dendritic cell （DC） vaccine in the patients with chronic hepatitis B（CHB） in vitro. Methods CIK cells from 30 patients with CHB were cultured in vitro, and were randomly divided into two groups, DC vaccine-treated group and the control group. After 14 days of culture, the percentages of CD3^＋ CD4^＋ T, CD3 ^＋ CD8^＋ T and CD3^＋ CD56^＋ T cells among CIKs were analyzed by flow cytometry. The levels of IL-12, IFN-＇y and IL-6 in cell culture supernatant was detected by ELISA. Results The percentages of CD3^＋ CD4^＋ T, CD3^＋ CD8^＋ T and CD3^＋ CD56^＋ T cells were 18.27%, 64. 36% and 20. 00% in CIKs in DC vaccine group, and 17. 79% （ P 〉 O. 05 ）, 54. 69% （ P 〈 0.01 ） and 13.39% （ P 〈 0. 01 ） in the control group, respectively. The perentage of CD3^＋ CD4^＋ T cells were similar between the two groups （ P 〉 O. 05 ）, but for the perentage of CD3^＋ CD8^＋ T and CD3^＋ CD56^＋ T cells were significantly different between the two groups（ t = 4. 130 and 5. 601 respectively, Ps 〈 0. 01 ）. The concentrations of IL-12, IL-4 and IFN-？ in supernatant were （ 177.82 ± 130.06）, （31.77 ±9. 52） and （86. 99 ±56. 30） ng/L in DC vaccine-treated group respectively, which were significantly different from those of （80. 83 ：t：50. 15） ng/L （t = 3. 811 ,P 〈0. 01 ）, （40. 33 ± 19. 74） ng/L（ t = 2. 141 ,P 〈 0. 05 ） and （42. 07 ：t： 19. 68 ） ng/L （ t = 4. 125, P 〈 0. 01 ） in the control group, respectively. Conclusion DC vaccine could enhance the killing function of CIK cells.
出处
《中国综合临床》
2012年第4期357-359,共3页
Clinical Medicine of China
基金
石家庄市科学研究与发展计划项目（06146613）
