摘要
目的观察补镁对2型糖尿病大鼠胰岛素受体(insulin receptor,IR)表达水平的影响。方法将高脂饲料喂养加链脲佐菌素注射方法诱发的2型糖尿病大鼠随机分为4个组,高、中、低剂量组在高脂饲料中分别加入氧化镁2000、1000、200mg/kg(以镁离子计),糖尿病对照组只喂饲高脂饲料,正常对照组喂饲普通饲料。动物自由进食,连续4周,用葡萄糖氧化酶法测定空腹尾尖血的血糖含量,腹主动脉取血用放免法测定血清胰岛素含量,用免疫组化法测定胰腺和骨骼肌组织IR表达水平。结果经Image-Pro Plus图像分析,高剂量组的胰腺和骨骼肌组织中IR表达水平分别为0.341±0.001和0.346±0.002,均较糖尿病对照组升高,而血糖水平则较糖尿病对照组降低,差异均有显著性意义(P<0.05)。结论镁补充可以提高2型糖尿病大鼠胰腺和骨骼肌组织中IR的表达水平,降低空腹血糖水平。
Objective To investigate the effects of magnesium intake on expression of insulin receptor in type 2 diabetes rats.Methods The models of type 2 diabetes rats were established by feeding with high-fat-diet and injecting streptozotocin（STZ）.Rats were randomly assigned to four groups.The high-fat-diets were administrated magnesium at a dose of 2000（high magnesium group）,1000（medium magnesium group）and200（low magnesium group）.Model control group was only fed with high-fat-diet.Normal control group was fed with common diet.Rats ate freely for four weeks.Fasting blood glucose was detected by glucose oxidase method..Insulin was detected by radio-immunity method.The expression levels of IR of pancreas and skeletal muscle were detected by immunohistochemistry method.Results The immunohistochemistry pictures were analyzed by Image-Pro Plus.Compared with model control group,the expression levels of IR of pancreas and skeletal muscle were 0.341±0.001 and 0.346±0.002,increased and fasting blood glucose decreased significantly in high magnesium group（P0.05）.Conclusion Magnesium intake may increase the expression levels of IR of pancreas and skeletal muscle,and decrease fasting blood glucose in type 2 diabetes rats.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第2期264-267,共4页
Journal of Hygiene Research
关键词
镁
2型糖尿病
胰岛素受体
magnesium
type 2 diabetes mellitus
insluin receptor
